Rabbit Antiserum To Human Albumin
Product is a lyophilized powder rabbit antiserum to human albumin and buffer salts.
This product is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques.
Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Immunoassays | Immunoelectrophoresis | Immunodiffusion | Immunoprecipitation
|Analysis Note||The total protein is measured using the Biuret procedure, with bovine albumin as a standard. Antibody titer is standardized by equivalence -point precipitation. Each antiserum is tested for specificity at a minimum of 80 mg/mL using immunoelectrophoresis.|
|Antibody Type||Polyclonal Antibody|
|Application Notes||This product is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques.|
|Concentration||Total Protein: 52.8 mg/mL; Antibody Protein: 8 mg/mL|
|Applications||Immunoassays | Immunoelectrophoresis | Immunodiffusion | Immunoprecipitation|
|Pack Size||2 mL|
|Physical Appearance||lyophilized powder|
|Preparation Method||The antiserum is prepared by hyperimmunizing rabbits with highly purified human albumin; antiserum is delipidated and made specific by solid-phase absorption. The resulting antiserum is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 µm filter, vialed and lyophilized.|
|Solubility||Reconstitution: Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material.|
|Specificity||It shows reactivity to human albumin; cross-reactivity to other species may exist. Antibody activity to other serum proteins is not present.|
|Sterility||Filtered through a .22 µm filter|
|Storage and Handling||Store lyophilized product at 2-8°C. Store reconstituted material no longer than 4 weeks at 2-8°C. For long-term storage; aliquot and freeze reconstituted product at -20°C or lower.|