Goat Antiserum To Human Secretory IgA

Goat Antiserum To Human Secretory IgA

MDL Number: MFCD00162412

Packaging size: 2 mL

SKU 0855080
Price:
$112.30
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Description

Product Description

Product is the lyophilized powder of goat antiserum to human secretory IgA and buffer salts.

Application Notes

This product is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. It is suitable for Ouchterlony double diffusion. The antibody was used for IgA quantification by ELISA in various studies. Antibody dilution of 1:4000 was used for passive hemagglutination inhibition assay.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Immunoassays | Precipitating antibody |Immunoprecipitation

Specifications
SKU 0855080
Analysis Note The total protein is measured using the Biuret procedure, with bovine albumin as a standard. Antibody titer is standardized by equivalence-point precipitation. Each antiserum is tested for specificity at a minimum of 80 mg/mL using immunoelectrophoresis.
Antibody Category Polyclonal
Antibody Type Polyclonal Antibody
Application Notes This product is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. It is suitable for Ouchterlony double diffusion. The antibody was used for IgA quantification by ELISA in various studies. Antibody dilution of 1:4000 was used for passive hemagglutination inhibition assay.
Conjugate Unconjugated
Formulation Details Lyophilized
Host Goat
Immunogen Purified human IgA
Applications Immunoassays | Precipitating antibody |Immunoprecipitation
MDL # MFCD00162412
Pack Size 2 mL
Personal Protective Equipment Eyeshields, Gloves, respirator filter
Physical Appearance Lyophilized powder
Preparation Method The antiserum is prepared by hyperimmunizing goats with highly purified human secretory IgA; antiserum is delipidated and made specific by solid-phase absorption. The resulting antiserum is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 µm filter, vialed and lyophilized.
Presentation Lyophilized powder
Research Areas Immunoassays
Solubility Reconstitution: Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material.
Species Reactivity Human; Secretory IgA
Specificity It shows reactivity to human secretory IgA; cross-reactivity to other species may exist. Antibody activity to human IgG; IgM; or non-immunoglobulin serum proteins is not present.
Sterility Filtered through a .22 µm filter
Storage and Handling Store lyophilized product at 2-8°C. Store reconstituted material no longer than 4 weeks at 2-8°C. For long-term storage; aliquot and freeze reconstituted product at -20°C or lower.
Trade Name Cappel™
Typical Working Concentration Suggested working dilution: 1:50 - 1:200 for Tissue Staining; 1:100 - 1:500 for Immunoblotting; 1:400 - 1:2,000 for ELISA
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.