Dino

Goat Affinity Purified Antibody To Human IgG (Whole Molecule)

Goat Affinity Purified Antibody To Human IgG (Whole Molecule)

MDL Number: MFCD00162452

Packaging size: 2 mg

SKU 0855070
Price:
$223.50
In Stock
Request a quote
Description

Product Description

Goat affinity purified antibody to human IgG (Whole Molecule) is goat affinity purified antibody to human IgG (whole molecule) and buffer salts.

Application Notes

IgG, Whole Molecule Polyclonal Antibody is used in immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections. May be used in quantitative precipitin assay. This product is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. It is also used for conjugation with enzymes, radiolabels, fluorochromes or other markers, for attachment to solid supports for use as an immunoadsorbant, as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining. These affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Immunoassays | Immunohistochemistry (Paraffin; Acetone-Fixed) | Immunofluorescence assays (IFA)

Specifications
SKU 0855070
Analysis Note The total protein is measured by absorbance (A280nm). Antibody titer is standardized by equivalence-point precipitation. Each affinity purified antibody is tested for purity at 10 mg/mL using immunoelectrophoresis.
Antibody Category Polyclonal
Antibody Type Polyclonal Antibody
Application Notes IgG, Whole Molecule Polyclonal Antibody is used in immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections. May be used in quantitative precipitin assay. This product is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. It is also used for conjugation with enzymes, radiolabels, fluorochromes or other markers, for attachment to solid supports for use as an immunoadsorbant, as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining. These affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.
Certifications The product is goat IgG.
Concentration Total Protein: 1.08 mg/mL; Active Antibody: 1.0 mg/mL
Conjugate Unconjugated
Formulation Details Liquid
Host Goat
Immunogen Human IgG
Applications Immunoassays | Immunohistochemistry (Paraffin; Acetone-Fixed) | Immunofluorescence assays (IFA)
Pack Size 2 mg
Physical Appearance Lyophilized powder
Preparation Method The affinity purified antibody is prepared from the specific goat IgG fraction by solid phase absorption. The resulting affinity purified antibody is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 μm filter, and vialed. No perservative has been added.
Presentation Affinity purified liquid
Product Families Description Goat affinity purified antibody to human IgG (Whole Molecule) is goat affinity purified antibody to human IgG (whole molecule) and buffer salts.
Purity Affinity Purified
Research Areas Immunoassays
Solubility Soluble in Water
Species Reactivity Human; (Whole Molecule)
Specificity Product shows reactivity to human IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG; some antibodies are expected to bind light chain sites common to all immunoglobulins.
Storage and Handling Store unopened product at 2-8°C. Store opened material no longer than 1-2 days at 2-8°C unless preservative is added. For long term storage; aliquot and store with 40% glycerol (v/v) at -20°C.
Typical Working Concentration Quantitative precipitin assay: 3.0 mg/mL, 1:50 - 1:200 for Tissue Staining, 1:100 - 1:500 for Immunoblotting, 1:400 - 2,000 for Elisa
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.