Goat Affinity Purified Antibody To Human IgA (Alpha Chain)

Goat Affinity Purified Antibody To Human IgA (Alpha Chain)

MDL Number: MFCD00162412

Packaging size: 1 mg

SKU 0855068
Price:
$157.30
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Description

Product Description

Product is goat affinity purified antibody to human IgA (α-chain) and buffer salts.

Application Notes

This product is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. This product is suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. This product is suitable for use as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining. It is also used for IgA quantification by ELISA in various studies. Antibody dilution of 1:4000 was used for passive hemagglutination inhibition assay. It may be used to locate and identify the α-chain of human IgA by immunocytochemical and immunohistochemical (IHC) techniques.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Immunoassays | Ouchterlony double diffusion | Immunohistochemistry

Specifications
SKU 0855068
Analysis Note The total protein is measured by absorbance (A280nm). Antibody titer is standardized by equivalence-point precipitation. Each affinity purified antibody is tested for purity at 10 mg/mL using immunoelectrophoresis. The product is goat IgG; no trace of albumin is detected.
Antibody Category Polyclonal
Antibody Type Polyclonal Antibody
Application Notes This product is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. This product is suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. This product is suitable for use as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining. It is also used for IgA quantification by ELISA in various studies. Antibody dilution of 1:4000 was used for passive hemagglutination inhibition assay. It may be used to locate and identify the α-chain of human IgA by immunocytochemical and immunohistochemical (IHC) techniques.
Concentration Total Protein: 1.1 mg/mL; Active Antibody: 1.0 mg/mL
Conjugate Unconjugated
Formulation Details Purified
Host Goat
Immunogen Purified human IgA
Applications Immunoassays | Ouchterlony double diffusion | Immunohistochemistry
MDL # MFCD00162412
Pack Size 1 mg
Personal Protective Equipment Eyeshields, Gloves, respirator filter
Physical Appearance Liquid
Preparation Method The affinity purified antibody is prepared from the specific goat IgG fraction by solid phase absorption. The resulting affinity purified antibody is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 µm filter, and vialed.
Presentation Affinity purified liquid
Purity Affinity Purified
Research Areas Immunoassays
Solubility Soluble in Water
Species Reactivity Human; (Alpha Chain)
Specificity It shows reactivity to human IgA; cross-reactivity to other species may exist. Antibody activity to IgG; IgM; or non-immunoglobulin serum proteins is not present.
Sterility Filtered through a .22 µm filter
Storage and Handling Store unopened product at 2-8°C. For long term storage; aliquot and store with 40% glycerol (v/v) at -20°C.
Trade Name Cappel™
Typical Working Concentration Suggested working dilution: 1:50 - 1:200 for Tissue Staining; 1:100 - 1:500 for Immunoblotting; 1:400 - 1: 2,000 for ELISA
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.