Goat F(Ab')2 Fragment To Human Kappa Chain (Bound)

Goat F(Ab')2 Fragment To Human Kappa Chain (Bound)

Packaging size: 2 mL

SKU 0855059
Price:
$259.70
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Description

Product Description

Product is the lyophilized powder of goat F(ab')2 fragment to human kappa chain (bound) and buffer salts.

Application Notes

Goat F(AB')2 fragment to Human (K) chain is use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. This product is suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. This product is suitable for use as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining. F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Immunoassays | Precipitating antibody |Immunoprecipitation

Specifications
SKU 0855059
Analysis Note The total protein is measured using the Biuret procedure, with bovine albumin as a standard. Antibody titer of the IgG fraction is standardized by equivalence-point precipitation. F(ab')<sub>2</sub> fragments are tested for purity and specificity at a minimum of 20 mg/mL using immunoelectrophoresis. The product is goat F(ab')<sub>2</sub>; no trace of albumin, whole molecule IgG or Fc is detected.
Antibody Category Polyclonal
Antibody Type Polyclonal Antibody
Application Notes Goat F(AB')<sub>2</sub> fragment to Human (K) chain is use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. This product is suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. This product is suitable for use as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining. F(ab')<sub>2</sub> fragments are recommended for staining of cells or tissues which contain Fc receptors.
Concentration Total Protein: 20.4 mg/mL; Antibody Protein: 8 mg/mL
Conjugate Unconjugated
Formulation Lyophilized
Host Goat
Applications Immunoassays | Precipitating antibody |Immunoprecipitation
Pack Size 2 mL
Physical Appearance Lyophilized powder
Preparation Method The F(ab')<sub>2</sub> fragment is prepared from the specific goat IgG fraction by limited digestion with pepsin, and purified by gel filtration or affinity chromatography. The resulting F(ab')<sub>2</sub> fragment is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 μm filter, vialed and lyophilized.
Presentation F(ab')2/Lyophilized
Research Areas Immunoassays
Solubility Reconstitution: Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material.
Species Reactivity Human; Kappa Chain (Bound)
Specificity F(ab')2 fragments are tested for purity and specificity at a minimum of 20 mg/ml using immunoelectrophoresis. The product is goat F(ab')2; no trace of albumin; whole molecule IgG or Fc is detected. It shows reactivity to bound kappa light chains; cross-reactivity to other species may exist. Because the product is directed against the kappa chains in Fab; some antibodies are expected to bind light chain sites common to all immunoglobulins. Antibody activity to free kappa light chains may be present. Antibody activity to human lambda Fab; free lambda light chains; or non-immunoglobulin serum proteins is not present.
Storage and Handling Store lyophilized product at 2-8°C. Store reconstituted material no longer than 1-2 days at 2-8°C unless preservative is added. For long term storage; aliquot and freeze reconstituted product at -20°C or lower.
Trade Name Cappel™
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.