Goat F(Ab')2 Fragment To Human IgM (5Fc µ)

Goat F(Ab')2 Fragment To Human IgM (5Fc µ)

Packaging size: 2 mL

SKU 0855055
Price:
$232.85
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Description

Product Description

Product is the lyophilized powder of goat F(ab')2 fragment to human IgM (5Fc μ) and buffer salts.

Application Notes

Goat F(AB')2 fragment to Human IgM is use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. This product is suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. This product is suitable for use as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining. F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. (Note: Affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals).

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Immunoassays | Precipitating antibody |Immunoprecipitation

Specifications
SKU 0855055
Analysis Note The total protein is measured using the Biuret procedure, with bovine albumin as a standard. Antibody titer of the IgG fraction is standardized by equivalence-point precipitation. F(ab')<sub>2</sub> fragments are tested for purity and specificity at a minimum of 20 mg/mL using immunoelectrophoresis. The product is goat F(ab')<sub>2</sub>; no trace of albumin, whole molecule IgG or Fc is detected.
Antibody Category Polyclonal
Antibody Type Polyclonal Antibody
Application Notes Goat F(AB')<sub>2</sub> fragment to Human IgM is use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. This product is suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. This product is suitable for use as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining. F(ab')<sub>2</sub> fragments are recommended for staining of cells or tissues which contain Fc receptors. (Note: Affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals).
Concentration Total Protein: 6.2 mg/mL; Antibody Protein: 3.5 mg/mL
Conjugate Unconjugated
Formulation Details Lyophilized
Host Goat
Applications Immunoassays | Precipitating antibody |Immunoprecipitation
Pack Size 2 mL
Physical Appearance Lyophilized powder
Preparation Method The F(ab')<sub>2</sub> fragment is prepared from the specific goat IgG fraction by limited digestion with pepsin, and purified by gel filtration or affinity chromatography. The resulting F(ab')<sub>2</sub> fragment is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 μm filter, vialed and lyophilized.
Presentation Antiserum/Lyophilized
Product Families Description Product is the lyophilized powder of goat F(ab')? fragment to human IgM (5Fc μ) and buffer salts.
Purity F(ab')<sub>2</sub> fragment
Research Areas Immunoassays
Solubility Reconstitution: Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material.
Species Reactivity Human; IgM (5Fc µ)
Specificity Product shows reactivity to human IgM; cross-reactivity to other species may exist. Antibody activity to human IgG; IgA; Fab or non-immunoglobulin serum proteins is not present.
Storage and Handling Store lyophilized product at 2-8°C. For long term storage; aliquot and freeze reconstituted product at -20°C or lower.
Trade Name Cappel™
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.