Dino

Goat F(Ab')2 Fragment To Human IgG (Whole Molecule)

Goat F(Ab')2 Fragment To Human IgG (Whole Molecule)

MDL Number: MFCD00162452

Packaging size: 2 mL

SKU 0855049
Price:
$147.65
In Stock
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Description

Product Description

Product is the lyophilized powder of goat F(ab')2 fragment to human IgG (whole molecule) and buffer salts.

Application Notes

Product is use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. It can be used for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. This product is suitable for use as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining. It is also used in Quantitative Precipitin Assay at concentration of 3.0-4.0 mg/mL in Indirect ELISA(1:80,000).

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Immunoassays | ELISA | Quantitative precipitin assay

Specifications
SKU 0855049
Analysis Note The total protein is measured using the Biuret procedure, with bovine albumin as a standard. Antibody titer of the IgG fraction is standardized by equivalence-point precipitation. F(ab')2 fragments are tested for purity and specificity at a minimum of 20 mg/mL using immunoelectrophoresis. The product is goat F(ab')2; no trace of albumin, whole molecule IgG or Fc is detected.
Antibody Type Polyclonal Antibody
Application Notes Product is use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. It can be used for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. This product is suitable for use as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining. It is also used in Quantitative Precipitin Assay at concentration of 3.0-4.0 mg/mL in Indirect ELISA(1:80,000).
Concentration Total Protein: 27.2 mg/mL; Antibody Protein: 10 mg/mL
Conjugate Unconjugated
Formulation Details Lyophilized
Host Goat
Applications Immunoassays | ELISA | Quantitative precipitin assay
Pack Size 2 mL
Personal Protective Equipment Eyeshields, Gloves, half-mask respirator, multi-purpose combination respirator cartridge
Physical Appearance Lyophilized powder
Preparation Method The F(ab')2 fragment is prepared from the specific goat IgG fraction by limited digestion with pepsin, and purified by gel filtration or affinity chromatography. The resulting F(ab')2 fragment is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 µm filter, vialed and lyophilized. No preservative has been added.
Presentation lyophilized
Product Families Description Product is the lyophilized powder of goat F(ab')2 fragment to human IgG (whole molecule) and buffer salts.
Recommended Replacement For 855057
Research Areas Immunoassays
Solubility Reconstitution: When reconstituted with 2.0 mL of deionized or distilled water, the product will have an antibody concentration of 10 mg/mL. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material.
Species Reactivity Human; (Whole Molecule)
Specificity It shows reactivity to human IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG; some antibodies are expected to bind light chain sites common to all immunoglobulins.
Sterility Filtered through a .22 µm filter
Storage and Handling Store lyophilized product at 2-8°C. For long term storage; aliquot and freeze reconstituted product at -20°C or lower.
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.