Rabbit IgG Fraction To Human Albumin

Rabbit IgG Fraction To Human Albumin

Packaging size: 2 mL

SKU 0855029
Price:
$199.10
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Description

Product Description

Product is the lyophilized powder of rabbit IgG fraction to human albumin and buffer salts.

Application Notes

This product is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. This product is suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. This product is suitable for use as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Immunoassays | Immunoelectrophoresis | Immunodiffusion | Immunoprecipitation

Specifications
SKU 0855029
Analysis Note The total protein is measured using the Biuret procedure, with bovine albumin as a standard. Antibody titer is standardized to an in-house standard by equivalence-point precipitation. Each IgG fraction is tested for purity and specificity at a minimum of 40 mg/mL using immunoelectrophoresis. The product is mostly rabbit IgG; no trace of albumin is detected.
Antibody Category Polyclonal
Antibody Type Polyclonal Antibody
Application Notes This product is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. This product is suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. This product is suitable for use as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining.
Concentration Total Protein: 15.1 mg/mL; Antibody Protein: 5 mg/mL
Conjugate Unconjugated
Formulation Lyophilized
Host Rabbit
Immunogen Human albumin
Applications Immunoassays | Immunoelectrophoresis | Immunodiffusion | Immunoprecipitation
Pack Size 2 mL
Physical Appearance Lyophilized powder
Preparation Method The IgG fraction is prepared from the specific rabbit antiserum by delipidation, ammonium sulfate fractionation and anion exchange chromatography. The resulting IgG fraction is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 µm filter, vialed and lyophilized.
Presentation Lyophilized(IgG)
Research Areas Immunoassays
Solubility Reconstitution: Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material.
Species Reactivity human
Specificity It shows reactivity to human albumin; cross-reactivity to other species may exist. Antibody activity to other serum proteins is not present.
Sterility Filtered through a .22 µm filter
Storage and Handling Store lyophilized product at 2-8°C. For long term storage; aliquot and store reconstituted product frozen at -20°C or lower.
Trade Name Cappel™
Typical Working Concentration Suggested working dilutions: 1:50 - 1:200 for Tissue Staining; 1:100 - 1:500 for Immunoblotting; 1:400 - 1:2,000 for ELISA/FIA
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.