Goat IgG Fraction To Human IgG Fab
Product is the lyophilized powder of goat IgG fraction to human IgG Fab and buffer salts. The IgG fraction is prepared from the specific goat antiserum by delipidation, ammonium sulfate fractionation and anion exchange chromatography. The resulting IgG fraction is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a a 0.22 µm filter, vialed and lyophilized.
This product is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques.
Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
|Antibody Type||Polyclonal Antibody|
|Application Notes||This product is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques.|
|Concentration||Total Protein: 29.3 mg/ml Antibody Protein: 8 mg/ml|
|Pack Size||5 mL|
|Physical Appearance||Lyophilized powder|
|Solubility||Soluble in Water|
|Species Reactivity||Human; IgG Fab|
|Specificity||The total protein is measured using the Biuret procedure; with bovine albumin as a standard. Antibody titer is standardized to an in-house standard by equivalence-point precipitation. Each IgG fraction is tested for purity and specificity at a minimum of 40 mg/ml using immunoelectrophoresis. The product is mostly goat IgG; no trace of albumin is detected. It shows reactivity to human Fab and IgG; cross-reactivity to other species may exist. Because the product is directed against Fab; some antibodies are expected to bind light chain sites common to all immunoglobulins. Antibody activity to Fc or non-immunoglobulin serum proteins is not present.|
|Storage and Handling||Store at 2-8°C.|
|Typical Working Concentration||Reconstitute product with 5.0 ml of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material.|