Purified Mouse Myeloma Protein IgG2B (Kappa) MOPC 141

Purified Mouse Myeloma Protein IgG2B (Kappa) MOPC 141

Packaging size: 1 mg

SKU 0850331
Price:
$212.05
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Description

Product Description

Purified mouse myeloma protein IgG2B (Kappa) is purified mouse IgG2b(j) and buffer. Mouse IgG2b is purified from clarified mouse MOPC 141 ascites using multi-step procedures which may include salt fractionation, gel filtration, ion-exchange chromatography, affinity chromatography, and immunoabsorption. The product is dialyzed into 0.02M TRIS, 0.15M sodium chloride, pH 8.1, filtered through a 0.22 µm filter and vialed.

Application Notes

This product is suitable for use as a positive control in antibody identification screening techniques and is recommended as an ELISA coating antigen and control.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Immunoassays | Positive control in antibody identification screening techniques

Specifications
SKU 0850331
Analysis Note The total protein is determined by absorbance at 280nm. The product contains 1 mg of mouse IgG2b. The product is tested for purity at 1.0 mg/mL using immunoelectrophoresis.
Antibody Type Monoclonal Antibody
Application Notes This product is suitable for use as a positive control in antibody identification screening techniques and is recommended as an ELISA coating antigen and control.
Certifications The product is mouse IgG2b.
Clone Name MOPC 141
Concentration Total Protein: 1.0 mg/mL; Volume: 1.0 mL
Conjugate Unconjugated
Formulation Purified
Host Mouse
Isotype IgG
Applications Immunoassays | Positive control in antibody identification screening techniques
Pack Size 1 mg
Physical Appearance Puried product in buffer
Preparation Method Mouse IgG2b is purified from clarified mouse MOPC 141 ascites using multi-step procedures which may include salt fractionation, gel filtration, ion-exchange chromatography, affinity chromatography, and immunoabsorption. The product is dialyzed into 0.02M TRIS, 0.15M sodium chloride, pH 8.1, filtered through a 0.22 μm filter and vialed. No preservative has been added.
Presentation Purified proteins are dialyzed into 0.01M Sodium Phosphate; 0.13M sodium chloride; pH 7.2.
Purity Affinity Purified
Research Areas Immunoassays
Solubility Soluble in Water
Specificity The total protein is determined by absorbance at 280nm. The product contains 1 mg of mouse IgG2b. The product is tested for purity at 1.0 mg/ml using immunoelectrophoresis. The product is mouse IgG2b; no trace of IgG1; IgG2a; IgG3; IgA; IgM or non-immunoglobulin serum proteins is detected.
Storage and Handling Store unopened product at -70°C. Store opened material no longer than a few days at 2-8°C unless sodium azide is added. For long-term storage; aliquot and freeze at -70°C.
Trade Name Cappel™
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.