Horseradish Peroxidase (Rz >3.0)

Horseradish Peroxidase (Rz >3.0)

Synonyms Horseradish peroxidase

CAS Number: 9003-99-0

EC Number: 232-668-6

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SKU 0836451-CF
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Description

Product Description

Horseradish peroxidase (HRP) is isolated from horseradish roots and belongs to the ferroprotoporphyrin group of peroxidases. HRP is a single chain polypeptide containing four disulfide bridges. It is a glycoprotein containing 18% carbohydrate. The carbohydrate composition consists of galactose, arabinose, xylose, fucose, mannose, mannosamine, and galactosamine depending upon the specific isozyme.

Application Notes

Horseradish peroxidase is used for production of peroxidase conjugated antisera. It is a widely used label for immunoglobulins in many different immunochemistry applications including ELISA, immunoblotting, and immunohistochemistry. HRP can be conjugated to antibodies by several different methods including glutaraldehyde, periodate oxidation, through disulfide bonds, and also via amino and thiol directed crosslinkers. HRP is the most desired label for antibodies, since it is the smallest and most stable of the three most popular enzyme labels (HRP, β-galactosidase, and alkaline phosphatase) and its glycosylation leads to lower non-specific binding.

Key Applications

Protein Labeling | Production of peroxidase conjugated antisera | Immunoblot | ELISA | Immunohistochemistry

Specifications
SKU 0836451-CF
Alternate Names Horseradish peroxidase
Application Notes Horseradish peroxidase is used for production of peroxidase conjugated antisera. It is a widely used label for immunoglobulins in many different immunochemistry applications including ELISA, immunoblotting, and immunohistochemistry. HRP can be conjugated to antibodies by several different methods including glutaraldehyde, periodate oxidation, through disulfide bonds, and also via amino and thiol directed crosslinkers. HRP is the most desired label for antibodies, since it is the smallest and most stable of the three most popular enzyme labels (HRP, β-galactosidase, and alkaline phosphatase) and its glycosylation leads to lower non-specific binding.
EC Number 232-668-6
Formulation Details Powder
Isoelectric Point Isozymes range from 3.0–9.0
Applications Protein Labeling | Production of peroxidase conjugated antisera | Immunoblot | ELISA | Immunohistochemistry
Physical Appearance brown to red brown color; salt-free; freeze-dried powder
Presentation powder
Product Families Description Horseradish peroxidase (HRP) is isolated from horseradish roots and belongs to the ferroprotoporphyrin group of peroxidases. HRP is a single chain polypeptide containing four disulfide bridges. It is a glycoprotein containing 18% carbohydrate. The carbohydrate composition consists of galactose, arabinose, xylose, fucose, mannose, mannosamine, and galactosamine depending upon the specific isozyme.
Protein or Enzyme Type Other Proteins, Enzymes Peptides
Solubility Soluble in distilled water or dilute buffer.
Source Horseradish Roots
Specific Activity ≥200 units/mg
Storage and Handling Best stored at -20°C. Once opened; redesiccate over silica gel under vacuum before returning to -20°C.
Trade Name Immuno™
Unit Definition The amount of enzyme causing the production of one milligram of purpurgallin in 20 seconds at 25 °C under the given assay conditions.