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Zymolyase, 20T from Arthrobacter luteus, 1 g

Zymolyase, 20T from Arthrobacter luteus, 1 g

Packaging size: 1 g

SKU 08320921
$555.90
In Stock
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Description

Product Description

Zymolyase-20T is an enzyme preparation from a submerged culture of Arthrobacter luteus which effectively lyses cell walls of viable yeast cells. This preparation is a lyophilized powder prepared by salting out from the culture fluid with ammonium sulfate and contains 20,000 units enzyme/g. The primary enzyme in this preparation responsible for lysis of viable yeast cells is ß-1,3-glucan laminaripentaohydrolase. It hydrolyzes glucose polymers at the ß-1,3-glucan linkages releasing laminaripentaose as the principal product.

Application Notes

Zymolyase 20T is used to lyse living yeast cell walls to produce protoplast or spheroplast of various strains of yeast cells. It may also be used for glucan hydrolysis. Zymolase-has a low solubility. Use as a suspension. If it is necessary to make a sterile enzyme solution of more than .5%, make a 2% enzyme stock solution by dissolving Zymolase -1T in a buffer solution (pH 7.5) which contains 5% glucose. To remove insoluble substances, filter through a sterile filter. Do not use Nitrocellulose filters. Dilute sterile enzyme suspension on to desired concentration.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Yeast Cell Culture | Used to lyse cell wall of yeast

Specifications
SKU 08320921
Application Notes Zymolyase 20T is used to lyse living yeast cell walls to produce protoplast or spheroplast of various strains of yeast cells. It may also be used for glucan hydrolysis. Zymolase-has a low solubility. Use as a suspension. If it is necessary to make a sterile enzyme solution of more than .5%, make a 2% enzyme stock solution by dissolving Zymolase -1T in a buffer solution (pH 7.5) which contains 5% glucose. To remove insoluble substances, filter through a sterile filter. Do not use Nitrocellulose filters. Dilute sterile enzyme suspension on to desired concentration.
Base Catalog Number 320921
Formulation Details Lyophilized
Pack Size 1 g
pH Optimum pH and temperature: pH 7.5, 35 °C for lysis of viable yeast cells; pH 6.5, 45 °C for hydrolysis of yeast glucan; pH Range: Stable through pH ranges 5-10
Protein or Enzyme Type Yeast Lytic Enzymes
Source Arthrobacter luteus
Specific Activity 20,000 units/g
Specificity The lytic spectrum of this enzyme includes organisms from the following genera: Ashbya; Candida; Debaryomyces; Eremothecium; Endomyces; Hansenula; Hanseniaspora; Kloekera; Kluyveromyces; Lipomyces; Metschikowia; Pichia; Pullularia; Torulopsis; Saccharomyces; Saccharomcopsis; Saccharomycodes; Schwannimomyces and others.
Unit Definition The amount of activity causing a decrease in absorbance at 800 nm of 30%.
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.