Proline Specific Endopeptidase (From Flavobacterium Meningosepticum)

Proline Specific Endopeptidase (From Flavobacterium Meningosepticum)

Synonyms Prolyl endopeptidase; Post-proline cleaving enzyme; Post-proline endopeptidase

CAS Number: 72162-84-6 Molecular Weight: ~78 kDa

Packaging size: 50 U

SKU 08320821
Price:
$1,101.70
In Stock
Request a quote
Description

Product Description

Proline Specific Endopeptidase specifically cleaves peptide bonds on the carboxy side of proline residues. Much slower hydrolysis is observed on the carboxy side of alanine residues. This enzyme is very close, in its properties, to a post-proline cleaving enzyme. The substrates have been found to have the general structure Y-Pro-X, where Y is a peptide or N-protected amino acid and X may be an amino acid, peptide, amide or ester.

Application Notes

Proline specific endopeptidase is useful for the determination of amino acid sequences of peptides and proteins containing proline residues. Proline specific endopeptidases may be useful in cancer, neurology and diabetes research. Prolyl endopeptidase (PEP) may be used as a drug target for neuropsychiatric diseases such as stress disorder, depression, and schizophrenia.

Key Applications

Cloning Expression | Determination of amino acid sequences of peptides and proteins containing proline residues

Specifications
SKU 08320821
Alternate Names Prolyl endopeptidase; Post-proline cleaving enzyme; Post-proline endopeptidase
Application Notes Proline specific endopeptidase is useful for the determination of amino acid sequences of peptides and proteins containing proline residues. Proline specific endopeptidases may be useful in cancer, neurology and diabetes research. Prolyl endopeptidase (PEP) may be used as a drug target for neuropsychiatric diseases such as stress disorder, depression, and schizophrenia.
Formulation Lyophilized
Isoelectric Point 9.1
Applications Cloning Expression | Determination of amino acid sequences of peptides and proteins containing proline residues
Molecular Weight ~78 kDa
Pack Size 50 U
pH Optimum pH 7.0
Physical Appearance Lyophilized powder
Presentation Lyophilized powder
Protein or Enzyme Type Molecular Biology Enzymes
Research Areas Ubiquitination
Source Flavobacterium meningosepticum
Specific Activity Not less than 5.0 U/mg material
Storage and Handling Stable for at least one year at 2-8°C in a desiccator.
Trade Name Immuno™
Unit Definition One unit causes the formation of one μmole of p-nitroaniline per minute under specific pH and temperature conditions. Enzyme Activity: One unit is the enzyme activity which produces 1 μmol p-nitroaniline per minute at 30 °C, pH 7.0, from Z-Gly-Pro-pNA. (Z: carbobenzoxy-)