Dl-Dithiothreitol

Dl-Dithiothreitol

Synonyms 1,4-Dithiothreitol; DTT; 1,4-Dithio-DL-threitol; Cleland Reagent; (±)-threo-1,4-Dimercapto-2,3-butanediol

CAS Number: 578517 Molecular Formula: C4H10O2S2

Beilstein Registry Number: 1719757 EC Number: 222-468-7 MDL Number: MFCD00004877

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SKU 04856126-CF
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Description

Product Description

Dl-Dithiothreitol

Application Notes

Dithiothreitol is useful for stabilizing sulfhydryl containing enzymes. Effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N'-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Sulfhydryl reducing agent||Blotting||Protein Purification

Specifications
SKU 04856126-CF
Alternate Names 1,4-Dithiothreitol; DTT; 1,4-Dithio-DL-threitol; Cleland Reagent; (±)-threo-1,4-Dimercapto-2,3-butanediol
Analysis Note Useful for stabilizing sulfhydryl-containing enzymes
Application Notes Dithiothreitol is useful for stabilizing sulfhydryl containing enzymes. Effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N'-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol.
Beilstein Registry Number 1719757
EC Number 222-468-7
Format powder
Grade Electrophoresis Grade
Half Life 1.4( pH 8.5 at 2 °C)
Hazard Statements H302-H315-H319-H335
Applications Sulfhydryl reducing agent||Blotting||Protein Purification
MDL # MFCD00004877
Molecular Formula C4H10O2S2
Personal Protective Equipment Dust mask , Eyeshields, Faceshields, Gloves
Physical Appearance White Powder
Product Families Description Dithiothreitol (DTT)
Product Overview Dl-Dithiothreitol
Purity ≥99.5%
RTECS Number EK1610000
Safety Symbol GHS07
Storage and Handling +4 °C
Typical Working Concentration Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.