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Dl-Dithiothreitol

Dl-Dithiothreitol

Synonyms 1,4-Dithiothreitol; DTT; 1,4-Dithio-DL-threitol; Cleland Reagent racemic; (±)-threo-1,4-Dimercapto-2,3-butanediol; Cleland’s reagent

CAS Number: 27565-41-9 Molecular Formula: C4H10O2S2

Beilstein Registry Number: 1719757 EC Number: 248-531-9 MDL Number: MFCD00004877

SKU 02194821-CF
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Description

Product Description

Dl-Dithiothreitol

Application Notes

DL-Dithiothreitol is used for stabilizing sulfhydryl containing enzymes. It is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N'-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Reducing Agent

Specifications
SKU 02194821-CF
Alternate Names 1,4-Dithiothreitol; DTT; 1,4-Dithio-DL-threitol; Cleland Reagent racemic; (±)-threo-1,4-Dimercapto-2,3-butanediol; Cleland’s reagent
Application Notes DL-Dithiothreitol is used for stabilizing sulfhydryl containing enzymes. It is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N'-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel.
Base Catalog Number 194821
Beilstein Registry Number 1719757
CAS # 27565-41-9
EC Number 248-531-9
Format Powder
Grade Molecular Biology Grade
Half Life .1 M KPO4 buffer (Half Life in hours):- pH 6.5 at 2 °C: 4, pH 7.5 at 2 °C: 1, pH 8.5 at 2 °C: 1.4, pH 8.5 at °C: 11, pH 8.5 at 4 °C: .2, pH 8.5 at 2 °C with addition of .1 mM Cu2+: .6, pH 8.5 at 2 °C with addition of 1. mM EDTA: 4
Hazard Statements H302-H315-H319-H335
Molecular Formula C4H10O2S2
Personal Protective Equipment Dust mask, Eyeshields, Faceshields, Gloves
Purity ≥99%
RTECS Number EK1610000
Safety Symbol GHS07
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.