Dimethyl Sulfoxide, Molecular Biology Reagent

Dimethyl Sulfoxide, Molecular Biology Reagent

Synonyms DMSO; Methyl sulfoxide; Methyl sulphoxide; Sulfinylbismethane

CAS Number: 67-68-5 Molecular Formula: C2H6OS Molecular Weight: 78.129 g/mol

Beilstein Registry Number: 506008 EC Number: 200-664-3 MDL Number: MFCD00002089

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SKU 02194819-CF
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Description

Product Description

Dimethyl Sulfoxide

Application Notes

Dimethyl Sulfoxide is used to enhance dermal absorption of many chemicals, as a solvent for many organic and inorganic compounds including fats, carbohydrates, dyes, resins, and polymers, in antifreeze or hydraulic fluids, as a cryopreservative for cell cultures, oxidation of thiols and disulfides to sulfonic acids, as a PCR cosolvent to help improve yields, especially in long PCR. DMSO is routinely used in polymerase chan reaction (PCR), amplification of cDNA libraries, DNA sequencing, column-loading buffers for poly(A)+ RNA selection, buffers for the transformation of competent E. coli, and transfection protocols.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

PCR Cosolvent

Specifications
SKU 02194819-CF
Application Notes Dimethyl Sulfoxide is used to enhance dermal absorption of many chemicals, as a solvent for many organic and inorganic compounds including fats, carbohydrates, dyes, resins, and polymers, in antifreeze or hydraulic fluids, as a cryopreservative for cell cultures, oxidation of thiols and disulfides to sulfonic acids, as a PCR cosolvent to help improve yields, especially in long PCR. DMSO is routinely used in polymerase chan reaction (PCR), amplification of cDNA libraries, DNA sequencing, column-loading buffers for poly(A)+ RNA selection, buffers for the transformation of competent E. coli, and transfection protocols.
Auto Ignition 419° F (USCG, 1999)
Beilstein Registry Number 506008
Boiling Point 372° F at 760 mm Hg (NTP, 1992)
Certifications Meets ACS specifications
Density 1.101 at 68° F (USCG, 1999)
Dielectric Constant 45 (Lit.)
EC Number 200-664-3
Flash Point 203° F (NTP, 1992)
Format liquid
Grade Molecular biology reagent
Applications PCR Cosolvent
Melting Point 65.3° F (NTP, 1992)
Molecular Formula C2H6OS
Molecular Weight 78.129 g/mol
Personal Protective Equipment Eyeshields, Full-face respirator, Gloves, Multi-purpose combination respirator cartridge, Respirator filter
Physical Appearance Liquid
Product Families Description Dimethyl sulfoxide
Product Overview Dimethyl Sulfoxide
Refractive Index n20D 1.479 (lit.)
RTECS Number PV6210000
Solubility greater than or equal to 100 mg/mL at 68° F (NTP, 1992)
Sterilization of Solutions To prepare sterile solutions use a teflon or nylon membrane to sterile-filter the DMSO - do not use a cellulose acetate membrane.
Storage and Handling Room Temperature; desiccate; store under nitrogen
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Vapor Density 2.71 (NTP, 1992) (Relative to Air)
Vapor Pressure 0.42 mm Hg at 68° F (NTP, 1992)