Dimethyl Sulfoxide, Molecular Biology Reagent
Product Description
Dimethyl Sulfoxide
Application Notes
Dimethyl Sulfoxide is used to enhance dermal absorption of many chemicals, as a solvent for many organic and inorganic compounds including fats, carbohydrates, dyes, resins, and polymers, in antifreeze or hydraulic fluids, as a cryopreservative for cell cultures, oxidation of thiols and disulfides to sulfonic acids, as a PCR cosolvent to help improve yields, especially in long PCR. DMSO is routinely used in polymerase chan reaction (PCR), amplification of cDNA libraries, DNA sequencing, column-loading buffers for poly(A)+ RNA selection, buffers for the transformation of competent E. coli, and transfection protocols.
Usage Statement
Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications
PCR Cosolvent
| SKU | 02194819-CF |
| Alternate Names | DMSO; Methyl sulfoxide; Methyl sulphoxide; Sulfinylbismethane |
| Application Notes | Dimethyl Sulfoxide is used to enhance dermal absorption of many chemicals, as a solvent for many organic and inorganic compounds including fats, carbohydrates, dyes, resins, and polymers, in antifreeze or hydraulic fluids, as a cryopreservative for cell cultures, oxidation of thiols and disulfides to sulfonic acids, as a PCR cosolvent to help improve yields, especially in long PCR. DMSO is routinely used in polymerase chan reaction (PCR), amplification of cDNA libraries, DNA sequencing, column-loading buffers for poly(A)+ RNA selection, buffers for the transformation of competent E. coli, and transfection protocols. |
| Auto Ignition | 419° F (USCG, 1999) |
| Base Catalog Number | 194819 |
| Beilstein Registry Number | 506008 |
| Boiling Point | 372° F at 760 mm Hg (NTP, 1992) |
| CAS # | 67-68-5 |
| Certifications | Meets ACS specifications |
| Density | 1.101 at 68° F (USCG, 1999) |
| Dielectric Constant | 45 (Lit.) |
| EC Number | 200-664-3 |
| Flash Point | 203° F (NTP, 1992) |
| Format | liquid |
| Grade | Molecular biology reagent |
| Applications | PCR Cosolvent |
| Melting Point | 65.3° F (NTP, 1992) |
| Molecular Formula | C2H6OS |
| Molecular Weight | 78.129 g/mol |
| Personal Protective Equipment | Eyeshields, Full-face respirator, Gloves, Multi-purpose combination respirator cartridge, Respirator filter |
| Physical Appearance | Liquid |
| Product Families Description | Dimethyl sulfoxide |
| Product Overview | Dimethyl Sulfoxide |
| Refractive Index | n20D 1.479 (lit.) |
| RTECS Number | PV6210000 |
| Solubility | greater than or equal to 100 mg/mL at 68° F (NTP, 1992) |
| Sterilization of Solutions | To prepare sterile solutions use a teflon or nylon membrane to sterile-filter the DMSO - do not use a cellulose acetate membrane. |
| Storage and Handling | Room Temperature; desiccate; store under nitrogen |
| Usage Statement | Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Vapor Density | 2.71 (NTP, 1992) (Relative to Air) |
| Vapor Pressure | 0.42 mm Hg at 68° F (NTP, 1992) |