2-Mercaptoethanol

2-Mercaptoethanol

Synonyms 2-Hydroxyethylmercaptan ; β-Mercaptoethanol; BME; Thioethylene glycol; 2-Hydroxy-1-ethanethiol; Monothioethyleneglycol ; 1-ethane-2-thiol; thioglycol ; 2-ME; b-ME; 2-thioethanol

CAS Number: 60-24-2 Molecular Formula: C2H6OS Molecular Weight: 78.129 g/mol

Beilstein Registry Number: 773648 EC Number: 200-464-6 MDL Number: MFCD00004890

Configure Your Product:

SKU 02194705-CF
Price:
$50.90
In Stock
Request a quote
Description

Product Description

2-Mercaptoethanol

Application Notes

2-Mercaptoethanol is used to reduce disulfide linkages in solubilizing proteins for gel electrophoresis (typically used in SDS-PAGE sample buffer at 5% concentration). Also it reduces excess oxidative polymerization of catalysts. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.In solution, 2-mercaptoethanol is readily oxidized in air to a disulfide, especially at alkaline pH. Because of this property, it is widely used to protect proteins, enzymes in particular, from becoming inactive. An excess of 2-mercaptoethanol (generally used at 0.01 M) will maintain the protein thiol groups in their reduced state.

Usage Statement

Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.

Key Applications

Reducing agent

Specifications
SKU 02194705-CF
Alternate Names 2-Hydroxyethylmercaptan ; β-Mercaptoethanol; BME; Thioethylene glycol; 2-Hydroxy-1-ethanethiol; Monothioethyleneglycol ; 1-ethane-2-thiol; thioglycol ; 2-ME; b-ME; 2-thioethanol
Application Notes 2-Mercaptoethanol is used to reduce disulfide linkages in solubilizing proteins for gel electrophoresis (typically used in SDS-PAGE sample buffer at 5% concentration). Also it reduces excess oxidative polymerization of catalysts. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.In solution, 2-mercaptoethanol is readily oxidized in air to a disulfide, especially at alkaline pH. Because of this property, it is widely used to protect proteins, enzymes in particular, from becoming inactive. An excess of 2-mercaptoethanol (generally used at 0.01 M) will maintain the protein thiol groups in their reduced state.
Auto Ignition 295°C
Beilstein Registry Number 773648
Boiling Point 315 to 316° F at 742 mm Hg (decomposes) (NTP, 1992)
Density 1.1143 at 68° F (NTP, 1992)
EC Number 200-464-6
Flash Point 165° F (NFPA, 2010)
Format liquid
Formulation The pure liquid (as supplied) is a 14.3 M liquid.
Grade Cell Culture Reagent
Hazard Statements H301 + H331-H310-H315-H317-H318-H373-H410
Applications Reducing agent
MDL # MFCD00004890
Melting Point -148° F (NTP, 1992)
Molecular Formula C2H6OS
Molecular Weight 78.129 g/mol
Personal Protective Equipment Faceshields, full-face respirator , Gloves, Goggles, multi-purpose combination respirator cartridge , respirator filter
Physical Appearance Liquid
Product Families Description 2-Mercaptoethanol
Product Overview 2-Mercaptoethanol
Purity ≥98%
Refractive Index n20/D 1.500 (Lit.)
RTECS Number KL5600000
Safety Symbol GHS05, GHS06, GHS08, GHS09
Solubility greater than or equal to 100 mg/mL at 68° F (NTP, 1992)
Storage and Handling Room Temperature
Usage Statement Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.
Vapor Density 2.69 (NTP, 1992) (Relative to Air)
Vapor Pressure 1 mm Hg at 68° F (NTP, 1992)