Isoamyl Alcohol

Isoamyl Alcohol

Synonyms Isopentyl alcohol; 3-Methyl-1-butanol; 3-Methylbutanol; Isopentyl alcohol; Isobutyl carbinol; Primary isoamyl alcohol; Fermentation amyl alcohol

CAS Number: 123-51-3 Molecular Formula: C5H12O Molecular Weight: 88.15 g/mol

Beilstein Registry Number: 1718835 EC Number: 204-633-5 MDL Number: MFCD00002934

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SKU 02194059-CF
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Description

Product Description

Isoamyl Alcohol

Application Notes

Isoamylalcohol is routinely used in molecular biology, especially in the purification of DNA. It is widely used in conjunction with phenol and chloroform (25:24:1 phenol : chloroform : isoamyl alcohol) for the removal of proteins from the nucleic acid solutions by extraction. Isoamyl alcohol is added to the phenol solution to help inhibit RNase activity and to help prevent the solubilization in the phenol phase of long RNA molecules with long poly(A) portions. It will also help in reducing the foaming during the extraction process.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

DNA purification||Conjugation

Specifications
SKU 02194059-CF
Alternate Names Isopentyl alcohol; 3-Methyl-1-butanol; 3-Methylbutanol; Isopentyl alcohol; Isobutyl carbinol; Primary isoamyl alcohol; Fermentation amyl alcohol
Application Notes Isoamylalcohol is routinely used in molecular biology, especially in the purification of DNA. It is widely used in conjunction with phenol and chloroform (25:24:1 phenol : chloroform : isoamyl alcohol) for the removal of proteins from the nucleic acid solutions by extraction. Isoamyl alcohol is added to the phenol solution to help inhibit RNase activity and to help prevent the solubilization in the phenol phase of long RNA molecules with long poly(A) portions. It will also help in reducing the foaming during the extraction process.
Auto Ignition 662° F (USCG, 1999)
Beilstein Registry Number 1718835
Boiling Point 270° F at 760 mm Hg (USCG, 1999)
Density 0.81 at 68° F (USCG, 1999)
EC Number 204-633-5
Flash Point 114° F (USCG, 1999)
Format liquid
Grade ACS
Hazard Statements H226-H332-H335
Applications DNA purification||Conjugation
MDL # MFCD00002934
Melting Point -179° F (NIOSH, 2016)
Molecular Formula C5H12O
Molecular Weight 88.15 g/mol
Personal Protective Equipment Eyeshields, Faceshields, full-face respirator , Gloves, multi-purpose combination respirator cartridge , respirator filter
Physical Appearance Clear; Colorless Liquid
Product Families Description Isoamyl alcohol
Product Overview Isoamyl Alcohol
Purity ≥98.5%
Refractive Index n20/D 1.406(lit.)
RTECS Number EL5425000
Safety Symbol GHS02, GHS07
Solubility 2 % at 57° F (NIOSH, 2016)
Storage and Handling Room Temperature
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Vapor Density 3.04 (AIR=1)
Vapor Pressure 28 mm Hg (NIOSH, 2016)