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Bilirubin Oxidase

Bilirubin Oxidase

Synonyms Bilirubin:oxygen oxidoreductase

CAS Number: 80619-01-8

MDL Number: MFCD00130650

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SKU 02190000-CF
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Description

Product Description

Bilirubin Oxidase

Application Notes

Bilirubin oxidase is used to degrade bilirubin. Bilirubin oxidase, from Myrothecium verrucaria, may be used to determine free hemoglobin in icteric specimens. It also has potential application in dye effluent decolorization and is a potential treatment for neonatal jaundice. It is used in diagnostic determination to determine two types of bilirubin, glucuronide-conjugated and unconjugated bilirubin separately. Also in various enzymatic assay using oxidase-peroxidase such as "Glucose oxidase system", "Cholesterol oxidase system", "Acyl coenzyme A oxidase system", and "Glycerol-3-phosphate oxidase system", the accuracy of the assay system can be increased as the interference of bilirubin is removed by oxidation of bilirubin in body fluid with bilirubin oxidase.

Usage Statement

Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.

Specifications
SKU 02190000-CF
Application Notes Bilirubin oxidase is used to degrade bilirubin. Bilirubin oxidase, from Myrothecium verrucaria, may be used to determine free hemoglobin in icteric specimens. It also has potential application in dye effluent decolorization and is a potential treatment for neonatal jaundice. It is used in diagnostic determination to determine two types of bilirubin, glucuronide-conjugated and unconjugated bilirubin separately. Also in various enzymatic assay using oxidase-peroxidase such as "Glucose oxidase system", "Cholesterol oxidase system", "Acyl coenzyme A oxidase system", and "Glycerol-3-phosphate oxidase system", the accuracy of the assay system can be increased as the interference of bilirubin is removed by oxidation of bilirubin in body fluid with bilirubin oxidase.
Biochemical Physiological Actions Bilirubin oxidase (BOD) is a multicopper oxidase that catalyzes the oxidation of bilirubin to biliverdin. Oxygen is the electron acceptor and is reduced to water. The enzyme is used for diagnostic analysis of bilirubin in serum during medical examinations of the serum. It has also been used as an enzymatic catalyst for the cathode of biofuel cells that work under neutral conditions. It is a monomeric protein with a molecular mass of 60 kDa and high reactivity at neutral pH. BOD participates in porphyrin and chlorophyll metabolism.
Format powder
Personal Protective Equipment Eyeshields, Gloves, respirator filter
Physical Appearance Light Bluish-Green Powder
Product Families Description Bilirubin oxidase
Product Overview Bilirubin Oxidase
Source Myrothecium species
Specific Activity ≥0.8 u/mg solid
Storage and Handling Store at -20 °C. Store Desiccated.
Unit Definition One unit is the amount of enzyme that oxidizes one micromole of bilirubin per minute at 37 °C, pH 8.4.
Usage Statement Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.