Endothelial Mitogen (50 mg)
Specifications
| SKU | 0216003850 |
| Application Notes | EM (also known as endothelial cell growth supplement) stimulates and supports the growth of vascular endothelial cells and keratinocytes. Additionally, it is used to improve the growth and supplement and/or reduce serum levels in endothelial cell, Balb/c 3T3, and hybridoma cell cultures. Endothelial Mitogen can be used for Human Mammalian Vascular Endothelial Cells at concentrations of 0.1-0.3mg/mL of serum supplemented media 0.01-0.1mg with heparin. Endothelial Mitogen can be used for Hybridoma Cell Cloning at concentrations of 0.025-0.15mg/ml of serum supplemented media. Endothelial Mitogen can be used for Balb/C-3T3 Cells at concentrations of 0.2mg/mL of serum supplemented media. |
| Concentration | 25-200 μg/mL |
| Formulation Details | Powder |
| Applications | Mammalian Cell Culture | Cell growth supplement |
| Pack Size | 50 mg |
| pH | 7.4 |
| Physical Appearance | Lyophilized orange powder |
| Presentation | The product was lyophilized from a solution of 0.1m NaCl and 5 mm NaH<sub>2</sub>PO<sub>4</sub>; pH 7.4 following filtration. | Lyophilized Powder |
| Protein or Enzyme Type | Growth Factors |
| Research Areas | Growth Factors Receptors |
| Solubility | Soluble in water, Reconstitute with 5-10 mL of sterile physiological saline mix gently and transfer to a sterile, screw-cap culture tube. Dilute to a final concentration of 50-300 μg/mL in the appropriate serum supplemented media. Solutions are stable 2 weeks at 4 °C and 1 month at -20 °C. |
| Source | Bovine hypothalamus |
| Sterility | Sterile | Filtration 0.1 micron membrane for removal of viable mycoplasma. |
| Storage and Handling | Store at -20°C. |