Epidermal Growth Factor 95%
Product Description
Epidermal growth factor or EGF is a growth factor isolated from mouse submaxillary glands that stimulates cell growth, proliferation, and differentiation by binding to its receptor EGFR. EGF is mitogenic for a variety of epidermal and epithelial cells, including fibroblasts, glial cells, mammary epithelial cells, vascular and corneal endothelial cells, bovine granulosa, rabbit chondrocytes, HeLa and SV40-3T3 cells.
Application Notes
EGF stimulates the proliferation and differentiation of a wide variety of cell types deriving both from ectoderm and mesoderm. In tissue culture, EGF can act to reduce or eliminate the requirement for serum and is often used in conjunction with other media additives and hormones, such as insulin, transferrin, and prostaglandin E1. Cellular metabolic effects of EGF include stimulation of ion fluxes, glucose transport, glycolysis, and synthesis of DNA, RNA and proteins.
Key Applications
Mammalian Cell Culture |Molecular Biology | Stimulates proliferation of cells
| SKU | 0216003501 |
| Alternate Names | EGF; Epidermal cell growth factor |
| Application Notes | EGF stimulates the proliferation and differentiation of a wide variety of cell types deriving both from ectoderm and mesoderm. In tissue culture, EGF can act to reduce or eliminate the requirement for serum and is often used in conjunction with other media additives and hormones, such as insulin, transferrin, and prostaglandin E1. Cellular metabolic effects of EGF include stimulation of ion fluxes, glucose transport, glycolysis, and synthesis of DNA, RNA and proteins. |
| EC Number | 263-468-7 |
| Formulation | Lyophilized |
| Grade | Tissue Culture Grade |
| Applications | Mammalian Cell Culture |Molecular Biology | Stimulates proliferation of cells |
| Molecular Weight | 6100 |
| Pack Size | 100 µg |
| Physical Appearance | White Solid |
| Preparation Method | The sample should be dissolved in sterile water or a sterile albumin supplemented buffer. Solutions of pure EGF should be aliquoted and lyophilized, while solutions containing albumin may be stored at -20 °C. |
| Presentation | Lyophilized from a solution of 0.01M sodium acetate | Solid |
| Purity | ≥95% |
| Source | Mouse Submaxillary Gland |
| Storage and Handling | Store at +4°C. Store dessicated. |