3,3',5,5'-Tetramethylbenzid

3,3',5,5'-Tetramethylbenzid

Synonyms BM blue; Sure Blue TMB; TMB Blotting Plus; TMB substrate; TMB

Beilstein Registry Number: 2808541 MDL Number: MFCD00007748

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SKU 02152346-CF
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$76.95
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Description

Product Description

TMB is a chromogenic substrate for Horse Radish Peroxidase (HRP). MP Biolomedicals TMB Solution is a convenient, non toxic and ready to use stable chromogen solution suitable for peroxidase and pseudo-peroxidase reactions in ELISA, it provides consistent performance and sensitivity to deliver reproducible results

Application Notes

Metabolism of the TMB substrate by HRP creates a soluble bluish reaction product that may be quantified by measuring absorbance at 370 or 665 nm. Reaction can be stopped by addition of 1 volume of 1N hydrocloric or sulfurc acid to yield a yellow reaction product that can be measured by absorbance at 450 nm (within one hour).

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

ELISA

Specifications
SKU 02152346-CF
Alternate Names BM blue; Sure Blue TMB; TMB Blotting Plus; TMB substrate; TMB
Application Notes Metabolism of the TMB substrate by HRP creates a soluble bluish reaction product that may be quantified by measuring absorbance at 370 or 665 nm. Reaction can be stopped by addition of 1 volume of 1N hydrocloric or sulfurc acid to yield a yellow reaction product that can be measured by absorbance at 450 nm (within one hour).
Beilstein Registry Number 2808541
Concentration 1.25 mM
Format Ready-to-use 1.25 mmol/liter stabilized chromogen solution.
Hazard Statements H315-H319-H335
Applications ELISA
MDL # MFCD00007748
Personal Protective Equipment Dust mask type , Eyeshields, Faceshields, Gloves
Physical Appearance Solution
RTECS Number DV2300000
Safety Symbol GHS07
Solubility Soluble in water
Storage and Handling +4°C; protect from light
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.