DNA extraction of mouse feces and intestinal content with SPINeasy DNA Kit for Feces (Cat. No. 116531050)
Difficulty of extracting DNA from the complex intestinal contents of mouse and their feces
The mouse genome is very similar to a human, making mouse genetic research particularly useful. In fact, 95 percent of all lab animals are mice and rats, according to the Foundation for Biomedical Research (FBR).
Studying their stool samples helps us understand gut fecal microbiota which can be indicative of many health cues. However, it is important to be able to extract pure, non-inhibitory DNA while maintaining high yield in short amount of time; One customer has difficulty with getting good quality and yield from the mouse fecal and intestinal content.
A sample of SPINeasy DNA Kit for Feces (Cat. No. 116531050) was offered by MP Biomedicals to address the poor results of their DNA extraction from mouse feces and intestinal contents.
Overview
Sample Name:
Mouse Fecal and intestinal contents
Sample Type:
Challenge :
DNA extraction results was not good
Featured Materials:
SPINeasy® DNA Pro Kit for Feces
High Performance kit for isolation of bacterial, fungal, plant and animal genomic DNA from soil and other environmental samples
Methods
Sample was prepared in order of protocol in accordance to product guidelines found here.
Outcome
Trial results data
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Trial Experience:
With SPINeasy DNA Kit for Feces, the operation was simple and the DNA extraction results was good.
The absorbance ratio 260/230 is higher than 1.0 which can meet the requirements of PCR and sequencing.
Conclusion
Key takeaways:
With SPINeasy DNA Kit for Feces, the protocol was simple to follow, DNA extraction results was good, absorbance ratio 260/230 was higher than 1 and it can meet the requirements of PCR and sequencing.
Looking To Try Our Extraction Kits?MP Biomedicals is now offering Free Trial Pack Application for the SPINeasy® DNA Pro Kit for Feces (5 Preps) to any valid research scientists. |
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