SPINeasy® DNA Pro Kit for Feces

Fecal samples display distinct processing challenges. The composition of fecal samples is largely dependent on diet which includes fibers, undigested particles, bilirubin, complex polysaccharides, and lipids. Those compounds impair the sample homogenization, decrease both the quantity and quality of the DNA extracted.

The SPINeasy® DNA Pro Kit for Feces was designed to tackle these challenges by providing an efficient way for rapidly isolating high-quality DNA from feces   samples. Irrespective of the complexities, samples are optimally homogenized by bead beating with the new Lysing Matrix YB and lysis Buffer SF1. Subsequent treatment with Buffer SF2 effectively removes humic acid and other contaminants. The chemistry included in Buffer SF3 enables the specific binding of DNA without co-purification of RNA, eliminating the need for RNase A treatment. DNA obtained from fecal samples showed no inhibition in PCR and was immediately ready-to-be use for downstream applications, including long fragment PCR, qPCR, and next-generation sequencing (16S and whole genome) without the need for a further inhibitor removal step.

Performance:-

Figure 1. The SPINeasy® DNA Pro Kit for Feces provides high-performance DNA from various fecal samples. A. The SPINeasy® DNA Pro Kit for Feces kit is able to handle a wide range of samples. The DNA yield, purity (260/280 and 260/230 ratio) and integrity were accessed using spectrophotometer and DNA gel, respectively. Each dot of the plot represents a single extraction. The horizontal bars indicate the median value. B. Comparison with the competitor Q kit. The DNA extracted using SPINeasy® DNA Pro Kit for Feces or competitor Q kits were analyzed. Representative virtual gels obtained following Agilent 4150 TapeStation analyses were displayed along with the DNA integrity value (DIN) and the size of the genomic DNA band in bp. C. Amplifiability. the absence of inhibitor in fecal samples obtained using SPINeasy® DNA Pro Kit for Feces kit was accessed using inhibitor-sensitive PCR and undiluted sample as well as quantitative PCR.

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Application Notes

The SPINeasy DNA Pro Kit for Feces allows for rapid isolation of high-quality, inhibitor-free genomic DNA from fecal samples. The kit effectively addresses common extraction challenges present with this sample type. Lysing Matrix tubes and specially designed lysis buffers remove common inhibitors and contaminants that co-purify with DNA. The final DNA product is ready for downstream applications, including long fragment PCR, qPCR, and next-generation sequencing, without the need for further inhibitor removal steps and RNase treatment.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Molecular Biology, Nucleic Acid Isolation and purification, PCR, qPCR, Gut microbiome analysis, NGS, shotgun sequencing