Dextran sulfate sodium salt, or DSS, is a synthetic sulfated branched polysaccharide derivative of dextran that has multiple uses in biomedical and clinical research (see table below). One important property of DSS is to triggers colitis in mice and rats when administered in drinking water. DSS binds to medium-chain-length fatty acids in the colon and induces intestinal inflammation. MP Premium Dextran Sulfate Sodium Salt (MW = 36,000–50,000) from MP Biomedicals is one of the most widely used product based on peer reviewed scientific publications. Over the past 15 years 3,000+ scientific publications have cited the use of our DSS.
Dextran sulfate is a polyanionic derivative of dextran produced by esterification of Dextran with chlorosulphonic acid. The sulfur content is approximately 17% which corresponds to an average of 1.9 sulfate groups per glucosyl residue of the dextran molecule.
Solubility: Soluble in water (100 mg/ml - clear to slightly hazy yellow solution. Higher molecular weight products may not be as soluble in water as the lower molecular weight products.). Aqueous solutions should be buffered with (e.g. with sodium bicarbonate) before autoclaving to prevent decomposition.
Typical Uses:
- Increases hybridization rate of nucleic acids - in the presence of 10% dextran sulfate the rate of reannealing of DNA in solution was increased by about 10 times. This observation was later extended to the hybridization of single or double-stranded probes to DNA or RNA immobilized on filter paper.9,16 The addition of 10% dextran sulfate may increase the rate of hybridization of randomly cleaved double-stranded DNA probes to immobilized nucleic acids by as much as 100 times.
- Shows immunologically relevant activities:
- enhancement and suppression of humoral immunity
- polyclonal activation of B-lymphocytes, stimulating even immature B cells6
- changes in thymocyte reactivity to lectins1
- inhibition of blood coagulation and platelet aggregation
- enhancement of blood fibrinolytic activity
- enhancement/suppression of cell-mediated immune responses
- Precipitates LDL and VLDL lipoproteins - in the presence of magnesium ions, dextran sulfate precipitates low-density lipoproteins from human serum leaving the high-density lipoproteins in the supernatant. Removal of lipoproteins by dextran sulfate precipitation may be useful in the purification of other materials such as beta2-glycoprotein
- Shows activity as an adjuvant
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[Webinar] Kopi & C Talk with RSC: Study of gut microbiota for inflammatory bowel disease (SG)
The guest for this episode of Kopi & C Talk is from MP Biomedicals, represented by Dr. Tan Yong Xing, a life science research scientist.
Find out more about how MP Biomedicals DSS induces physiological responses that mimic IBD in the research against colorectal cancer.
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Application Notes
Dextran sulfate sodium (DSS) is one of the most common and effective compounds used for inducing ulcerative colitis in animals. The DSS colitis model has also been used extensively to study colon cancer developing in relation to colonic inflammation, such as that occurring in patients with long-standing ulcerative colitis.
MP Biomedicals offers the gold standard of colitis model creation reagents - colitis grade dextran sulfate sodium salt (36,000-50,000) and colonic carcinogen azoxymethane (AOM).
Because of its high efficacy, MP Biomedical DSS is one of the most widely used DSS products in the scientific literature. Over the past 19 years more than 10,000 scientific publications have cited the use of MP Biomedicals’ DSS products and reported successful animal model establishment.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Acute/ Chronic Ulcerative Colitis Model Induction, Chronic Colitis Associated Colon Cancer Model Induction
SKU | 02160110-CF |
Alternate Names | DSS, Dextran Sodium Sulfate |
Application Notes |
Dextran sulfate sodium (DSS) is one of the most common and effective compounds used for inducing ulcerative colitis in animals. The DSS colitis model has also been used extensively to study colon cancer developing in relation to colonic inflammation, such as that occurring in patients with long-standing ulcerative colitis. MP Biomedicals offers the gold standard of colitis model creation reagents - colitis grade dextran sulfate sodium salt (36,000-50,000) and colonic carcinogen azoxymethane (AOM). Because of its high efficacy, MP Biomedical DSS is one of the most widely used DSS products in the scientific literature. Over the past 19 years more than 10,000 scientific publications have cited the use of MP Biomedicals’ DSS products and reported successful animal model establishment. |
Base Catalog Number | 160110 |
Boiling Point | 310 ° C |
CAS # | 9011-18-1 |
Grade | Colitis Grade |
Melting Point | 92 ° C |
Molecular Weight | 36,000 - 50,000 Da |
Personal Protective Equipment | Eyeshields, Gloves, respirator filter |
pH | (1% aqueous solution) 5-7.11 |
References |
Kinchen, J. Structural Remodeling of the Human Colonic Mesenchyme in Inflammatory Bowel Disease. Cell. 2018, 175, 372-386. Mahalhal , A. Oral iron exacerbates colitis and influences the intestinal microbiome, PLOS ONE. 2018, 13, e0202460. Dokoshi, T. Hyaluronidase inhibits reactive adipogenesis and inflammation of colon and skin. JCI Insight. 2018, 3, e12307. Zhu, H. RNA virus receptor Rig-I monitors gut microbiota and inhibits colitis-associated colorectal cancer. J. Exp. Clin. Cancer Res. 2017, 36, 2. Monticelli, L. IL-33 promotes an innate immune pathway of intestinal tissue protection dependent on amphiregulin–EGFR interactions. PNAS. 2015, 112, 10762-10767. |
RTECS Number | HH9290000 |
Solubility | 100 mg/mL (decreases with increasing of MW) |
Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
Vapor Pressure | 2.6 x 10-4 mm Hg at 25 ° C (Estimated) |