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Western Blot Workflow

Protein Extraction & Loading >Ships in One Business Day

From RIPA Lysis Buffer to Fluorescent Loading Buffers, our portfolio of high-quality reagents and buffers are ideal for initial protein extraction and PAGE loading. Combine with MP Bio’s loading controls and epitope tag antibodies to accurately quantify protein expression levels and verify the specificity of a protein of interest for Western blot analysis. Skip the gel staining step and visualize proteins post-electrophoresis with our Quick Fluorescent Loading Buffers and Fluorescent Protein Marker.


Category

Product

Cat. No.

RIPA Lysis Buffer

RIPA buffer with Triton X-100, 500 mL

04822792

Protein Quantification

BCA Protein Assay Kit

08W00021

Inhibitor Cocktails

Protease and Phosphatase Inhibitor Cocktail-10X

08W00017

Phosphatase Inhibitor Cocktail-10X

08W00018

Loading Buffers

Quick Fluorescent Loading Buffer (SDS-PAGE) - 3X

08W00013

Quick Fluorescent Loading Buffer (Native-PAGE) - 3X

08W00014

Protein Loading Buffer (SDS-PAGE) - 5X

08W00015

Protein Loading Buffer (Native-PAGE) -5X

08W00016

Protein Ladders

Fluorescent Protein Marker, 250 µL

08W00009

Prestained Triple Color Protein Ladder

08L100025-CF

Prestained Molecular Weight Standard, Dual Color (0.5 mL)

08W00027

PAGE Analysis >Ships in One Business Day

MP Bio now offers a comprehensive collection of pre-cast tris-glycine and bis-tris gels compatible with Bio Rad's Mini Protean Tanks, as well as running buffers for high-resolution protein separation. Achieve rapid electrophoresis with our Ultra-Fast Running Buffer, designed to shorten run times to about 20 minutes. Improve the overall experimental efficiency of electrophoresis using a combination of our precast gels, Quick Fluorescent Loading Buffer, Fluorescent Protein Marker, and Ultra-Fast Running Buffers.


Category

Product

Cat. No.

Running Buffers

Tris-Glycine Running Buffer for SDS-PAGE (Powder)

08W00010

Tris-Glycine Running Buffer for Native PAGE (Powder)

08W00011

Ultra-Fast Running Buffer for SDS-PAGE (Powder)

08W00012

MOPS SDS Running Buffer for Bis-Tris Gels (Powder)

08W00025

MES SDS Running Buffer for Bis-Tris Gels (Powder)

08W00026

Precast Gels

Precast Gel Plus Tris-Gly 10%, 10 Wells

08W00001

Precast Gel Plus Tris-Gly 10%, 15 Wells

08W00002

Precast Gel Plus Tris-Gly 12%, 10 Wells

08W00003

Precast Gel Plus Tris-Gly 12%, 15 Wells

08W00004

Precast Gel Plus Tris-Gly 15%, 10 Wells

08W00005

Precast Gel Plus Tris-Gly 15%, 15 Wells

08W00006

Precast Gel Plus Tris-Gly 4 - 20%, 10 Wells

08W00007

Precast Gel Plus Tris-Gly 4 - 20%, 15 Wells

08W00008

Precast Gel Plus, Bis-Tris, 4-20% Acrylamide, 10 Wells

08W00022

Precast Gel Plus, Bis-Tris, 4-20% Acrylamide, 12 Wells

08W00023

Precast Gel Plus, Bis-Tris, 4-20% Acrylamide, 15 Wells

08W00024

Gel Staining Solution

Ultra-Blue Fast PAGE Gel Staining Solution

08X10001

Western Blot Reagents >Ships in One Business Day

Our Western blot assay reagents include solutions to simplify the process and improve results. Western Stripping Buffer enables the reuse of membranes for multiple probing, saving both time and cost. In addition, OneStep Western Blocker and Signal Enhancer provides blocking and primary and secondary antibody hybridization in one step, while also enhancing signal development with HRP or AP substrates. This three-in-one solution is an ideal alternative for researchers seeking to streamline the Western blot procedure and minimize laborious steps.


Category

Product

Cat. No.

Blocker and Signal Enhancer

OneStep Western Blocker and Signal Enhancer (Protein-Free)

08X100002

Membrane Wash

Tris-Buffered Saline, TBS (Powder)

08W00020

ECL Substrate

Ultra-Sense Femto-Plus Western ECL Substrate

08X100003

WB Stripping

Western Stripping Buffer

08W00019

Resources

FastGroâ„¢ Fully Chemically Defined FBS Replacement

Triple Color Precision Protein Ladder (10 - 180 kDa)

Ready to use three color protein standard with 10 pre-stained proteins for monitoring protein separation

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Immunology & Cell Biology

Ultra-Blue Fast PAGE Gel Staining Solution

A ready to use protein staining solution for SDS-PAGE gels that enhances detection and eliminates the need for destaining

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Antibiotics for Cell Culture

OneStep Western Blocker and Signal Enhancer (Protein-Free)

Provides blocking and primary and secondary hybridization in one step while enhancing signal development with HRP or AP substrates.

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Challenges in Plant Research

Ultra-Sense Femto-Plus Western ECL Substrate

Our Luminol-based substrate is designed for HRP-conjugated primary & secondary antibodies to detect target proteins that are too small for typical ECL substrates.

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