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DNA extraction from marine sediment samples with SPINeasy DNA Kit for Soil (Cat. No.116530050)
Feeling under water from the difficulty of extracting good quality genomic DNA from marine environment
The isolation of good quality and quantity of DNA from microorganisms in marine sediment can be difficult because of the unique characteristic of the marine microorganisms and the marine environment.
The characteristic of marine microorganisms is interesting and greatly differs from the terrestrial microorganisms in term of both physiological and metabolic features. For example, the marine microorganisms are halophilic and are tolerant to low temperature and high pressure.
At the same time, the access to genomic DNA of microorganisms in marine environment is limited by low biomass turnover and low microbial activity because of extreme living condition and extreme energy limitation, which is scarcely available only as luminous energy. Furthermore, most microorganisms typically stay dormant as endospores.
The researcher has attempted many times with different brand of DNA extraction kits to extract DNA from the microorganisms in the marine sediment, but the yield and purity of the extracted DNA were low and did not meet the downstream experiment requirement.
The researcher tried SPINeasy DNA extraction for Soil, when offered by MP Biomedical, but failed on the first run of the experiment. MP Biomedical discussed with the researcher on the sample features and then recommended some adjustments to the protocol, including sample pre-treatment.
The results were satisfactory.
Overview
Sample Name:
Marine Sediment
Sample Type:
Featured Materials:
SPINeasy DNA Pro Kit for Soil
High Performance kit for isolation of bacterial, fungal, plant and animal genomic DNA from soil and other environmental samples
Methods
Sample was prepared in order of protocol in accordance to product guidelines found here.
The researcher adjusted the protocol as recommended, and repeated the DNA extraction with SPINeasy DNA Extraction kit of Soil (Cat No. 116530050), starting with lysis and homogenization steps with FastPrep 24 5G and lysing matrix. This time a good yield and purity was achieved which could meet the downstream experiment requirement.
Outcome
Trial result of different samples extracted with SPINeasy DNA Kit for Soil
| Sample ID | Conc.(ng/μl)h | A260/280 | A260/230 |
|---|---|---|---|
| W1 | 44.1 | 1.89 | 1.8 |
| W2 | 38.6 | 1.88 | 1.79 |
| S1 | 14.4 | 1.96 | 1.33 |
| S2 | 4.8 | 2.05 | 1.89 |
W1, W2 – Water samples
S1, S2 – Sediment samples
Conclusion
Key takeaways:
Based on MP company’s technical advice, we optimized the experiment conditions, and both the DNA yield and purity were improved. The DNA extracted from the marine sediment can meet downstream experiment requirements.
The researcher was satisfied with the results and has already made SPINeasy DNA extraction kit his choice for future study.
Looking To Try Our Extraction Kits?MP Biomedicals is now offering Free Trial Pack Application for the SPINeasy DNA Pro Kit for Soil (5 Preps) to any valid research scientists. |
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