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The ABCs of Lysing Matrices
With a wide range of bead sizes, shapes, and compositions, MP Bio offers a dependable lysing matrix for every application.
Size
The smaller the particles used in the grinding media, the smaller the average particle size and the smaller the lowest-limiting particle size produced during pulverization. Matrix particle size should be selected based upon the size of the particles you wish to obtain in your lysate.
Shape
The shape of the grinding media is a major determining factor in how cells are disrupted. Dull media, such as spherical beads, utilize cascade impaction (hammering) as the main force for cell lysis. Sharp and angular shaped media will primarily generate mechanical shear forces (chopping and cutting) which can quickly open difficult cell walls, grind fibrous or elastic animal tissue, or crack spores or oocytes. Shear forces are preferable when isolating stable molecules such as DNA, stable proteins, structural polysaccharides and small molecules or metabolites. RNA and certain easily denatured proteins can be quickly degraded by shear forces, so care needs to be taken when using angular media. For isolation of these molecules, smooth impactor grinding media can be much more forgiving.
Hardness, Density, and Composition
The composition determines two very important qualities: hardness and density, both of which are inherent physical properties derived from the molecular composition of the matrix particle. The hardness must be greater than that of the sample being pulverized, with higher hardness values being more effective at disrupting hard and brittle cell membranes. Hardness and density values help optimize lysis efficiency while preserving the integrity of the analytes of interest.
Choose from a wide variety of high-quality lysing matrix options
Lysing Matrix C
Matrix Composition: 1 mm silica spheres
- Yeast and Fungi (including spores)
- Algae
2ml 4.5ml 15ml 50ml 96-well Bulk
Lysing Matrix M
Matrix Composition: 1/4 inch ceramic beads
- All Samples
- Tough Tissues
- Skeletal Muscle
- Lung
- Heart
- Bone
- Seeds
- Spores
2ml 4.5ml 15ml 50ml 96-well Bulk
Lysing Matrix Tubes
Top Lysing Matrix Accessories
Resources
Brochure
Sample Preparation Brochure
Find a world of dependable sample preparation products for generating high-quality DNA, RNA, and proteins—FastPrep systems, lysing matrix tubes, kits, and more.
Download
Brochure
Environmental Brochure
From sample preparation to molecular analysis, find end-to-end solutions for working with environmental samples.
Download
Brochure
Lysing Matrix Selection Guide
Choose the perfect Lysing Matrix for any sample type in seconds
Learn More
Brochure
Nucleic Acid Extraction
Explore how MPBio can assist you in isolating DNA and RNA from your samples.
Learn More