MagBeads FastDNA Kit for Forensics

The MagBeads FastDNA Kit for Forensics utilizes advanced silica-coated magnetic bead technology to deliver high-performance DNA extraction from a wide range of forensic casework samples. Compared to traditional spin-column methods, the magnetic bead-based workflow enhances DNA recovery efficiency, particularly from low-template and degraded samples, while minimizing sample loss and contamination risk. The kit consistently provides high DNA yield and purity, with no detectable inhibition in downstream applications. 
The purification process is streamlined and automation-friendly, eliminating  centrifugation during the bind, wash, and elution steps and enabling easy integration with manual or automated platforms. DNA extracted from simulated and real forensic casework samples using this kit is of high integrity and can be directly used for downstream applications such as PCR/qPCR and STR analysis. The MagBeads FastDNA Kit for Forensics ensures robust and reliable STR profiling performance, making it an ideal solution for maximizing DNA recovery from challenging or limited forensic samples.

Performance
The MagBeads FastDNA Kit for Forensics has been extensively validated using sample types commonly encountered in forensic casework, including bloodstains, tape (to collect fingerprint), hair, nail, buccal swabs, cigarette butts, etc. 
Comparable DNA band intensities and qPCR amplification results were obtained across all four extraction formats, indicating that the extraction method did not significantly affect DNA recovery or downstream PCR performance.  

Figure 1: Comparison of four DNA extraction formats.
(A) Agarose gel electrophoresis of DNA extracted from buccal swab samples showing comparable DNA band intensities across all extraction formats. (B) qPCR amplification results of buccal swab and tape (with fingerprint) samples demonstrating comparable amplification performance across all four extraction formats
 

Figure 2: Real-time PCR for STR loci CSF1PO and DXS10134 using DNA Extracted from simulated casework samples.
Compared to Kit M, the MagBeads FastDNA Kit for Forensics constantly showed either similar or lower Ct value among all sample types and targets.

Figure 3: STR Profile Comparison Between the MagBeads FastDNA Kit for Forensics and Competitor Kit P
As shown in Figures 3A–C, the MagBeads FastDNA Kit for Forensics generated stronger and clearer STR profiles, with sharp, distinct peaks across the STR loci for all three sample types. In contrast, Competitor Kit P produced predominantly background noise with limited allele detection, particularly for the fingerprint and hair samples. These results demonstrate the ability of the MagBeads FastDNA Kit for Forensics to generate clear, high-quality STR profiles from these forensic sample types.  

Figure 4: STR Analysis of DNA Extracted from Pooled Whole Blood Using the MagBeads FastDNA Kit for Forensics
In addition, as shown in Figure 4, DNA extracted from pooled whole blood samples using the MagBeads FastDNA Kit for Forensics generated an STR profile with more than two peaks at multiple loci due to the presence of DNA from multiple donors.

Application Notes

MagBeads FastDNA Kit for Forensics is designed for the extraction of genomic DNA from a wide range of forensic evidence samples, delivering high-quality DNA suitable for STR profiling, PCR/qPCR, and other forensic molecular applications.

Usage Statement

Research Use Only (RUO)

Key Applications

STR profiling, PCR, qPCR