Sodium bicarbonate solution (7.5%) sterile-filtered, BioReagent, suitable for cell culture

Sodium Bicarbonate is buffer commonly used for maintaining the pH of cell culture medium in the presence of 4–10% carbon dioxide. In addition to buffering, sodium bicarbonate provides some nutritional benefit, while rarely displaying any cell toxicity. Formulated as 75 g per liter of water, Sodium Bicarbonate, 7.5% Solution is perfect for supplementing dry powder medium during reconstitution. The final Sodium Bicarbonate concentration used in the medium depends on the media formulation and the carbon dioxide concentration used in the incubator.

Application Notes

Research Use Only

Key Applications

Sodium bicarbonate solution has been used: as a component of Hanks′ buffered salt solution (HBSS) and recording medium for culturing of tissue explants from rats or mice as a component of Dulbecco′s modified Eagle′s medium (DMEM) to culture mice tissue explants as a component of Minimum Essential Media (MEM) to study the cytotoxicity against human colorectal adenocarcinoma cells and mammary gland adenocarcinoma