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How Mycoplasma Contamination Can Derail Your Research
Mycoplasma is a hidden threat to research, disrupting inflammation pathways and impairing cell metabolism, growth, and gene expression. These effects can lead to unreliable data, missed deadlines, and increased costs. Our Technical Note explains how mycoplasma affects the cells and various detection methods. Click now to protect your research from unseen threats.
Learn MoreOvercoming Mycoplasma Contamination: Detection, Prevention, and Removal
Mycoplasma contamination poses a significant challenge across cell culture, bioproduction, and clinical research, often leading to inaccurate results and compromised integrity. These antibiotic-resistant pathogens often evade detection, disrupting vital processes and skewing experimental outcomes. A robust strategy that integrates prevention, timely detection, and effective removal is essential for maintaining high research standards.
Shop All Mycoplasma Contamination SolutionsMycoplasma Prevention: The First Step in Effective Mycoplasma Management
Effective mycoplasma management begins with prevention. Adhering to Good Laboratory Practices (GLP), including mycoplasma-specific training, awareness, and rigorous cleaning & disinfecting protocols, can significantly reduce risk to your research. Incorporate MP Biomedicals' Mycoplasma Removal Agent (MRA) in your cultures and processes as a preventive measure — this proactive approach helps safeguard your research, resources, and reputation.
| Category | Target | Use Site | Suggested Frequency | Product Name |
|---|---|---|---|---|
| Antibiotics | Mycoplasma | Supplement cell culture media as antibiotic at 0.1 µg/mL | During cell culture maintenance and expansion | Mycoplasma Removal Agent (MRA) |
| Disinfectants | Mycoplasma, viruses, bacteria, fungi, and molds | PCR stations, benches, mycoplasma detection work area and tools | Before myco-detection assays and as needed | Myco-Out Mycoplasma Removal Spray |
| Tissue culture hoods, centrifuges, pipettes, tube holders, refrigerators, benches | Weekly to monthly | LabCare Cell Culture Room Antimicrobial Spray | ||
| Incubators | Weekly | LabCare Incubator Antimicrobial Spray | ||
| Stainless steel, glass, PTFE and other plastics, porcelain, ceramic | Constantly, to support aseptic techniques | Ethyl Alcohol, 140 Proof (70%) | ||
| Mycoplasma, bacteria, fungi, and algae | Water baths | Every 2 weeks | LabCare Water Bath Disinfectant Solution | |
| Incubator water pans | Every 1–3 months | LabCare Incubator Water Disinfectant Solution | ||
| Detergents | Mycoplasma and other bacterial particles, bioaccumulation, soil, proteins, oils, lipids, blood, tissue, pigments, fermentation residues, gels, starches | Stainless steel, glass, PTFE and other plastics, porcelain, ceramic | As needed and through routine cleaning | 7X® Cleaning Solution |
| ES-7X Cleaning Solution | ||||
| 7X-O-Matic Cleaning Solution | ||||
| ES-7X-O-Matic Cleaning Solution |
Mycoplasma Detection: Verify Your Contamination Prevention
Routine mycoplasma testing is crucial for validating your prevention strategy. Our PCR, LAMP, and enzymatic test kits offer rapid, highly sensitive detection suitable for routine monitoring in bioprocessing and mammalian cultures. Combining these methods reduces the risk of false results, providing the most dependable outcomes.
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| Product | Myco-Visible Mycoplasma qPCR Detection Kit |
Myco-Visible Mycoplasma PCR Detection Kit |
Myco-Visible Mycoplasma Rapid Test Kit |
Myco-Visible Mycoplasma LAMP Detection Kit |
| Key Features | EP/JP/USP compliant High specificity High sensitivity |
High sensitivity High specificity Rapid PCR-based detection |
Low detection limit High sensitivity Rapid detection |
Visual confirmation No plate reader or thermal cycler needed |
| Cat No. | 0930515-CF | 0930514-CF | 0930509-CF | 093050601 |
| Method | qPCR | PCR | LAMP + Lateral Flow | LAMP |
| Controls | Positive, Negative, Internal |
Positive, Negative, Internal |
Positive, Negative | Positive, Negative |
| Species Detected | >140 | >200 | >100 | 90 |
| Limit of Detection | 10 CFU/mL 10 fg gDNA |
10 CFU/mL 10 fg gDNA |
10 CFU/mL 10 fg gDNA |
10 CFU/mL 10 fg gDNA |
| Regulatory Compliance | Available upon request | Meets regulatory framework | N/A | N/A |
| Time to Results | 2.5 hours | ~3 hours | ~45 min | ~60 min |
| Instrument | qPCR Machine Heat Block |
Thermal Cycler Heat Block Electrophoresis System |
Microcentrifuge Heat Block |
Heat Block |
| Result Indicator | Ct value | Gel Bands | Lateral Flow Band | Solution Color Change |
| LEARN MORE | LEARN MORE | LEARN MORE | LEARN MORE |
Mycoplasma Removal: Act Quickly to Restore Cell Culture Integrity
When mycoplasma invades, prompt action is essential. MP Biomedicals' Mycoplasma Removal Agent (MRA) offers an advanced solution. As a quinolone antibiotic derivative, MRA specifically targets and inhibits mycoplasma DNA gyrase, removing multiple mycoplasma species within a week. With low cytotoxicity, proven efficacy at 0.5 µg/mL, and recognition in over 550 scientific studies, MRA ensures the accuracy and integrity of your research.
Mycoplasma Removal Agent: Restore Your Cultures and Eliminate Contamination
- Eliminates multiple mycoplasma species within a week
- Recognition in 550+ scientific publications
- Effective treatment at just 0.5 μg/mL
Mycoplasma Complete Solution
A comprehensive, all-in-one solution for reliable mycoplasma detection and control. Combines Myco qPCR and Myco LAMP for fast, sensitive, and accurate results.
Explore Complete Solution
Comprehensive Mycoplasma Contamination Control
Mycoplasma can silently compromise your research. Learn how to prevent, detect, and remove contamination while staying compliant with regulatory standards.
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Mycoplasma Contamination: The Impact on Cell-Based Research
Even the most controlled experiments can be compromised by mycoplasma. See how it influences cell-based research and why routine monitoring is critical.
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Strategies for Preventing Mycoplasma Contamination in Cell Cultures
Controlling mycoplasma starts with robust prevention. Learn how aseptic techniques, validated disinfectants, and quarantine protocols keep cultures clean.
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Comprehensive Guide to Mycoplasma Detection in Cell Cultures
From culture-based tests to PCR and enzymatic assays, discover the pros and cons of each detection method and how to implement them in your lab.
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Mycoplasma Removal from Contaminated Cell Culture, Tissues, or Products
Contamination doesn't have to mean starting over. Explore scientifically backed strategies to eliminate mycoplasma from contaminated cultures.
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Regulatory Compliance in Mycoplasma Management
Regulatory agencies demand strict mycoplasma testing and control to ensure product safety. Discover key compliance requirements and best practices.
Request Tech NoteFrequently Asked Questions
A: Mycoplasma are a group of gram-negative bacteria that lack a cell wall, making them resistant to many common antibiotics. They can contaminate cell cultures, leading to altered cell metabolism, gene expression, and even cell death. Contaminated cultures produce unreliable experimental data, making mycoplasma a significant concern in research and bioproduction.
A: Mycoplasma contamination is often asymptomatic, meaning your cells may look healthy despite being contaminated. Testing is the only reliable method to detect mycoplasma. Please refer to the table above to find the detection kit best suited to your requirements.
A: Common sources include cross-contamination from infected cell lines, contaminated reagents, lab personnel, and aerosolized particles. To minimize risk, maintain good laboratory practices, regularly test cultures, and use products like Myco-Out Mycoplasma Removal Spray or LabCare series to disinfect the workspace.
A: Yes. MP Biomedicals' Mycoplasma Removal Agent can help eliminate mycoplasma while preserving cell viability. Regular monitoring is essential to ensure contamination does not recur.
A: MP Biomedicals' Mycoplasma Removal Agent is specifically formulated for mycoplasma, ensuring high efficacy with minimal toxicity to your cultures. Unlike broad-spectrum antibiotics, it targets mycoplasma without affecting your cells' growth and function, making it suitable for both eliminating existing contamination and helping prevent future contamination during long-term cell culture maintenance.
A: It’s recommended to test all cultures at least once a month, or more frequently if you suspect contamination. MP Biomedicals' Mycoplasma Rapid Detection Kit is suitable for quick, fast detection when you need a simple check, while PCR-based kits can be used when more comprehensive molecular detection is required.
A: Use LabCare Water Bath Disinfectant Solution or LabCare Incubator Water Disinfectant Solution to effectively eliminate mycoplasma from water baths and incubators. These solutions are designed to be safe for equipment while providing long-lasting antimicrobial protection.
A: Yes, Mycoplasma Removal Agent can be used alongside other antibiotics if needed. However, it is specifically designed to target mycoplasma, so additional antibiotics may not be necessary unless other bacterial contaminants are present.