2-Mercaptoethanol, electrophoresis grade, 500 g

Name: 2-Mercaptoethanol, electrophoresis grade, 500 g
SKU: 04806445
Price: 7795 INR

₹7,795.00

In stock

SKU: 04806445

GST Rate: 18%

HSN Code: Not Available

Synonyms

β-Mercaptoethanol; 2-Hydroxyethylmercaptan; BME; Thioethylene glycol; 2-Hydroxy-1-ethanethiol; Monothioethyleneglycol

CAS Number:

60-24-2

Molecular Formula:

C₂H₆OS

Molecular Weight:

78.13

Beilstein Registry Number:

773648

EC Number:

200-464-6

MDL Number:

MFCD00004890

Packaging size:

500 g

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Specifications

SKU	04806445
Alternate Names	β-Mercaptoethanol; 2-Hydroxyethylmercaptan; BME; Thioethylene glycol; 2-Hydroxy-1-ethanethiol; Monothioethyleneglycol
Applications	Solubilizing proteins for gel electrophoresis
Application Notes	2-Mercaptoethanol is used to reduce disulfide linkages in solubilizing proteins for gel electrophoresis (typically used in SDS-PAGE sample buffer at 5% concentration). Also reduces excess oxidative polymerization of catalysts. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure. In solution, 2-mercaptoethanol is readily oxidized in air to a disulfide, especially at alkaline pH. Because of this property, it is widely used to protect proteins, enzymes in particular, from becoming inactive. An excess of 2-mercaptoethanol (generally used at 0.01 M) will maintain the protein thiol groups in their reduced state.
Base Catalog Number	806445
Beilstein Registry Number	773648
Boiling Point	157 °C (Lit.)
CAS #	60-24-2
Density	1.114 g/mL at 25 °C (Lit.)
EC Number	200-464-6
Flash Point	68 °C/154.4 °F (Lit.)
Grade	Electrophoresis grade
Melting Point	<-50 °C (Lit.)
Molecular Formula	C₂H₆OS
Molecular Weight	78.13
Pack Size	500 g
Personal Protective Equipment	Faceshields, full-face respirator, Gloves, Goggles, multi-purpose combination respirator cartridge, respirator filter
pH	5.0 (0.1M in water)(Lit.)
Purity	≥98%
RTECS Number	KL5600000
Safety Symbol	GHS05, GHS06, GHS08, GHS09
Solubility	Miscible with water, alcohol, ether and benzene. Solutions containing even trace levels (nanomolar) of some metal salts (especially copper (II) or cobalt(II)) are unstable at pH > 5 and progressively less stable at higher pH values. Most buffers (especially phosphate) contain enough metal salts to lead to substantial loss of -SH within 1-2 days. The addition of EDTA helps to stabilize solutions. If diluted product needs to be stored it is recommended to store at pH 6-7 with EDTA (0.05 mM) at 2-8 °C for no more than 2 to 3 days; however, we highly recommend adding neat 2-mercaptoethanol as needed to the sample.
Usage Statement	Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Vapor Density	2.69 (vs air)(Lit.)
Vapor Pressure	1 mmHg (20 °C)(Lit.)