Anti-human Kappa Chain (bound) goat IgG fraction, peroxidase-conjugated , 2 mL

Product Description

Peroxidase-Conjugated Goat IgG Fraction To Human Kappa Chain (Bound) is the lyophilized powder of horseradish peroxidase (HRP)-conjugated goat IgG fraction to human kappa light chain (bound) and buffer salts.

Application Notes

Peroxidase-Conjugated Goat IgG Fraction To Human Kappa Chain (Bound) is suitable for use as a reagent in enzyme immunoassays (EIA), cell and immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections, cell and tissue labeling (for electron microscopy), and blot immunostaining.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Immunohistochemistry | Immunoblotting |ELISA

Specifications

SKU	0855233
Alternate Names	Anti-Human
Antibody Type	Polyclonal Antibody
Application Notes	Peroxidase-Conjugated Goat IgG Fraction To Human Kappa Chain (Bound) is suitable for use as a reagent in enzyme immunoassays (EIA), cell and immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections, cell and tissue labeling (for electron microscopy), and blot immunostaining.
Base Catalog Number	55233
Concentration	Total Protein: 5.8 mg/mL; Titer: 1:181;000
Conjugate	HRP
Formulation Details	Lyophilized
Host	Goat
Immunogen	Human kappa light chains
Isotype	Polyclonal IgG
Pack Size	2 mL
Preparation Method	Antibody and highly purified HRP (Rz>3.0) are conjugated under defined conditions to obtain optimally labeled product. Conjugated protein is purified by salt fractionation. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 µm filter, vialed and lyophilized.
Purity	Purified
Solubility	Reconstitution: Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Do not add sodium azide. Avoid vigorous shaking of the reconstituted material.
Species Reactivity	Human, Kappa Chain (Bound)
Specificity	It shows reactivity to human kappa Fab; cross-reactivity to other species may exist. Because the product is directed against the kappa chains in Fab; some antibodies are expected to bind light chain sites common to all immunoglobulins.
Typical Working Concentration	Dilution Recommended: 1:500 - 1:4,000 for Tissue Staining; 1:1,000 - 1:8,000 for Immuno Blotting; 1:2,000 - 1:16,000 for ELISA/FIA
Uniprot Number	IGKC_HUMAN (P01834)
Usage Statement	Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.