Product is the lyophilized powder of goat IgG fraction to human IgG Fc and buffer salts.
Application Notes
Goat IgG fraction to Human IgG Fc is use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. It is suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. It is also suitable for use as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals).
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Immunoassays | Immunoelectrophoresis (IE) | Immunodiffusion techniques
| SKU | 0855014 |
| Antibody Type | Polyclonal Antibody |
| Application Notes | Goat IgG fraction to Human IgG Fc is use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. It is suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. It is also suitable for use as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals). |
| Base Catalog Number | 55014 |
| Concentration | Total Protein: 34.1 mg/mL; Antibody Protein: 5 mg/mL |
| Conjugate | Unconjugated |
| Format | lyophilized |
| Formulation Details | Powder |
| Grade | Molecular Grade |
| Host | Goat |
| Pack Size | 2 mL |
| Preparation Method | The IgG fraction is prepared from the specific goat antiserum by delipidation, ammonium sulfate fractionation and anion exchange chromatography. The resulting IgG fraction is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 μm filter, vialed and lyophilized. |
| Solubility | Reconstitution: Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material. |
| Species Reactivity | Human |
| Specificity | Product shows reactivity to human Fc and IgG; cross-reactivity to other species may exist. Antibody activity to Fab; IgA; IgM or non-immunoglobulin serum proteins is not present. |
| Typical Working Concentration | Typical working dilution: 1:50 - 1:200 for Tissue Staining; 1:100 - 1:500 for Immunoblotting; 1:400 - 2,000 for Elisa |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
![MP COA [55014] lot ID: 00606](https://www.mpbio.com/static/version1715349838/frontend/Mpbio/default/en_US/images/document.png "MP COA [55014] lot ID: 00606")
