M9 minimal medium salts
SKU: 113037012-CF
M9 Minimal Salts are a commonly used base for preparing a defined synthetic medium for the growth of most E. coli host cells. The powder is reconstituted in sterile purified water, (or water with melted purified agar added.) Additional nutrients, such as glucose or other carbon source, casamino acids, vitamins, or antibiotics are added when the solution cools and before plates are poured or media is inoculated with bacteria.
Application Notes
M9 Minimal medium salts are suitable for the preparation of M9 Minimal Medium for use in propagation of E. coli and plasmid amplification. The resulting M9 medium can be used for maintaining positive selection pressure on plasmids coding for genes to produce essential substances such as amino acids and vitamins and for maintenance of F’-containing bacteria strains for M13 propagation.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Cell culture |Proteomics |Purification and Detection |Recombinant Protein Expression and Analysis |Bacterial Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Genetic Manipulation |Growth and Maintenance of M13 phage & other filamentous ssDNA bacteriophages
| SKU | 113037012-CF |
| Alternate Names | M9 Minimal Media |
| Application Notes | M9 Minimal medium salts are suitable for the preparation of M9 Minimal Medium for use in propagation of E. coli and plasmid amplification. The resulting M9 medium can be used for maintaining positive selection pressure on plasmids coding for genes to produce essential substances such as amino acids and vitamins and for maintenance of F’-containing bacteria strains for M13 propagation. |
| Base Catalog Number | 3037012 |
| Components | Contents/L of 5x solution: 33.9 g Na2HPO4 15 g KH2PO4 2.5 g NaCl, 5 g NH4Cl |
| Format | Powder |
| Formulation Details | 33.9 g Na2HPO4; 15 g KH2PO4; 2.5 g NaCl; 5 g NH4Cl = 56.4 g/L of a 5x solution. |
| Grade | Molecular Biology Certified™ |
| Media Type | M9 Minimal Medium |
| Personal Protective Equipment | Personal Protective Equipment Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: Pvc or other plastic material gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice. |
| pH | Acceptable pH range is: 6.5 - 7.2 |
| Preparation Method | Media Preparation:Reagents are tested and chosen for their ability to promote optimum growth of E. coli when combined in M9 Minimal Medium Salt formulations. Chosen reagents are combined in the proper proportions according to the formulation listed in the Certificate of Analysis and thoroughly blended for a uniform distribution. |
| Solubility | Soluble in water |
| Sterilization of Solutions | A 56.4 g sample of M9 Minimal Salts was dissolved in water to 1 liter, the pH adjusted to 7.4, if needed, and the solution autoclaved to make a sterile 5X concentrate. |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
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