Anti-mouse immunoglobulins (IgG, IgA, IgM) goat IgG fraction, peroxidase-conjugated, 2 mL

Product Description

Product is the lyophilized powder of horseradish peroxidase (HRP)-conjugated goat IgG fraction to mouse immunoglobulins (IgG,IgA,IgM) and buffer salts.

Application Notes

Peroxidase conjugated Goat IgG fraction to mouse immunoglobulins(IgG, IgA, IgM) is used as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. (Note: F(ab')₂ fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies or their fragments are recommended to avoid non-specific binding from inherent antibodies of host animals). Detection of antibodies in sera of T. gondii-infected mice was done by ELISA using peroxidase-conjugated anti-mouse polyvalent immunoglobulin antibody as the secondary.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Immunohistochemistry | Immunoblotting |ELISA

Specifications

SKU	0855556
Antibody Type	Polyclonal Antibody
Application Notes	Peroxidase conjugated Goat IgG fraction to mouse immunoglobulins(IgG, IgA, IgM) is used as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. (Note: F(ab')₂ fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies or their fragments are recommended to avoid non-specific binding from inherent antibodies of host animals). Detection of antibodies in sera of T. gondii-infected mice was done by ELISA using peroxidase-conjugated anti-mouse polyvalent immunoglobulin antibody as the secondary.
Base Catalog Number	55556
Concentration	Total Protein: 11.4 mg/mL; Titer: 1:200;000
Conjugate	HRP
Format	lyophilized
Formulation Details	Lyophilized
Grade	Molecular Grade
Host	Goat
Pack Size	2 mL
Preparation Method	Antibody and highly purified HRP (Rz >3.0) are conjugated under defined conditions to obtain optimally labeled product. Conjugated protein is purified by salt fractionation. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 μm filter, vialed and lyophilized. No preservative has been added.
Purity	Purified
Solubility	Soluble in water
Species Reactivity	Mouse
Specificity	Product shows reactivity to mouse immunoglobulins (IgG;IgA;IgM); cross-reactivity to other species may exist. Antibody activity to non-immunoglobulin serum proteins is not present.
Usage Statement	Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.