Anti-human IgM (5Fc µ) goat IgG fraction, peroxidase-conjugated , 2 mL

Product Description

Product is the lyophilized powder of horseradish peroxidase (HRP)-conjugated goat IgG fraction to human IgM (5Fc μ) and buffer salts.

Application Notes

Peroxidase-Conjugated Goat IgG fraction to Human IgM is used as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. (Note: F(ab')₂ fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies are recommended to avoid non-specific binding from inherent antibodies of host animals).

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Immunohistochemistry | Immunoblotting |ELISA

Specifications

SKU	0855228
Antibody Type	Polyclonal Antibody
Application Notes	Peroxidase-Conjugated Goat IgG fraction to Human IgM is used as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. (Note: F(ab')₂ fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies are recommended to avoid non-specific binding from inherent antibodies of host animals).
Base Catalog Number	55228
Concentration	Total Protein: 10.0 mg/mL; Titer: 1:185;000
Conjugate	HRP
Format	lyophilized
Formulation Details	Lyophilized
Grade	Molecular Grade
Host	Goat
Pack Size	2 mL
Preparation Method	Antibody and highly purified HRP (Rz>3.0) are conjugated under defined conditions to obtain optimally labeled product. Conjugated protein is purified by salt fractionation. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.01% thimerosal, adjusted to standard titer, filtered through a 0.22 μm filter, vialed and lyophilized.
Purity	Purified
Solubility	Reconstitution: Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Do not add sodium azide. Avoid vigorous shaking of the reconstituted material.
Species Reactivity	Human
Specificity	It shows reactivity to human IgM; cross-reactivity to other species may exist. Antibody activity to IgG; IgA; Fab; or non-immunoglobulin serum proteins is not present.
Typical Working Concentration	Dilution reccomendations: 1:500 - 1:4,000 for Tissue Staining; 1:1,000 - 1:8,000 for Immuno Blotting; 1:2,000 - 1:16,000 for ELISA/FIA.
Usage Statement	Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.