Anti-human IgG F(Ab')2 goat IgG fraction, peroxidase-conjugated , 2 mL

Product Description

Peroxidase-Conjugated Goat IgG Fraction to Human IgG F(ab')₂ is the lyophilized powder of horseradish peroxidase (HRP)-conjugated goat IgG fraction to human IgG F(ab')₂ and buffer salts.

Application Notes

Peroxidase-Conjugated Goat IgG Fraction to Human IgG F(ab')₂ is suitable for use as a reagent in enzyme immunoassays (EIA), cell and immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections, cell and tissue labeling (for electron microscopy), and blot immunostaining. (Note: F(ab')₂ fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies or their fragments are recommended to avoid non-specific binding from inherent antibodies of host animals. Also used in Immunohistochemistry, EIA and Western blots.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Immunohistochemistry | Immunoblotting |ELISA

Specifications

SKU	0855224
Antibody Type	Polyclonal Antibody
Application Notes	Peroxidase-Conjugated Goat IgG Fraction to Human IgG F(ab')₂ is suitable for use as a reagent in enzyme immunoassays (EIA), cell and immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections, cell and tissue labeling (for electron microscopy), and blot immunostaining. (Note: F(ab')₂ fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies or their fragments are recommended to avoid non-specific binding from inherent antibodies of host animals. Also used in Immunohistochemistry, EIA and Western blots.
Base Catalog Number	55224
Concentration	Total Protein: 5.9 mg/mL; Titer: 1:192;000
Conjugate	HRP
Format	lyophilized
Formulation Details	Lyophilized
Grade	Molecular Grade
Host	Goat
Immunogen	Purified human IgG
Pack Size	2 mL
Preparation Method	Antibody and highly purified HRP (Rz>3.0) are conjugated under defined conditions to obtain optimally labeled product. Conjugated protein is purified by salt fractionation. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.01% thimerosal, adjusted to standard titer, filtered through a 0.22 µm filter, vialed and lyophilized.
Purity	Purified
Solubility	Reconstitution: Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Do not add sodium azide. Avoid vigorous shaking of the reconstituted material.
Species Reactivity	Human
Specificity	It shows reactivity to human IgG F(ab')<sub>2</sub>; cross-reactivity to other species may exist. Because the product is directed against F(ab')<sub>2</sub>; some antibodies are expected to bind light chain sites common to all immunoglobulins.
Typical Working Concentration	Immunohistochemistry: 1:500 - 1:5000; EIA and Western blots: 1:5000 - 1:100,000. Dilution recommended: 1:500 - 1:4,000 for Tissue Staining; 1:1,000 - 1:8,000 for Immuno Blotting; 1:2,000 - 1:16,000 for ELISA/FIA
Usage Statement	Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.