Anti-human IgG goat F(Ab')2 fragment, fluorescein-conjugated (whole molecule), 2 mL

Product Description

Fluorescein-conjugated Goat F(ab')₂ fragment to Human IgG is the fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat F(ab')₂ fragment to human IgG (whole molecule) and buffer salts.

Application Notes

Fluorescein-conjugated goat F(ab')₂ fragment to human IgG is suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. F(ab')₂ fragments are recommended for staining of cells or tissues which contain Fc receptors. (Note: Affinity purified antibodies and their F(ab')₂ fragments are recommended to avoid non-specific binding from the inherent antibodies of host animals. The antibody may also be used in FACS-based assays (1:200).

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Immunoassays | Immunohistochemistry (Paraffin; Acetone-Fixed; 4% PFA Frozen) | Immunofluorescence assays (IFA) | Flow Cytometry (FACS)

Specifications

SKU	0855180
Antibody Type	Polyclonal Antibody
Application Notes	Fluorescein-conjugated goat F(ab')₂ fragment to human IgG is suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. F(ab')₂ fragments are recommended for staining of cells or tissues which contain Fc receptors. (Note: Affinity purified antibodies and their F(ab')₂ fragments are recommended to avoid non-specific binding from the inherent antibodies of host animals. The antibody may also be used in FACS-based assays (1:200).
Base Catalog Number	55180
Concentration	Total Protein: 28.1 mg/mL; Antibody Protein: 10 mg/mL
Conjugate	FITC
Format	lyophilized
Formulation Details	Lyophilized
Grade	Molecular Grade
Host	Goat
Immunogen	Pooled normal human serum
Pack Size	2 mL
Personal Protective Equipment	Eyeshields, Gloves, half-mask respirator, multi-purpose combination respirator cartridge
Preparation Method	Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6 mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer and vialed.
Purity	Purified
Solubility	Soluble in Water
Species Reactivity	Human
Specificity	This product shows reactivity to human IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG; some antibodies are expected to bind light chain sites common to all immunoglobulins. Antibody activity to non-immunoglobulin serum proteins is not present.
Typical Working Concentration	Dilution Recommendations: Direct immunofluorescence (1:16); ANA-indirect immunofluorescence (1:32 or 1:64); FACS-based assays (1:200); 1:100 - 1:400 for Tissue Staining; 1:250 - 1:1,000 for Immuno Blotting; 1:1,000 - 1:4,000 for ELISA/FIA
Usage Statement	Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.