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3,3',5,5'-Tetramethylbenzide Liquid Substrate

3,3',5,5'-Tetramethylbenzide Liquid Substrate
€71.70

Key features and details

TMB is a chromogenic substrate for Horse Radish Peroxidase (HRP). MP Biolomedicals TMB Solution is a convenient, non toxic and ready to use stable chromogen solution suitable for peroxidase and pseudo-peroxidase reactions in ELISA, it provides consistent performance and sensitivity to deliver reproducible results

SKU: 02152346-CF

Synonyms
BM blue; Sure Blue TMB; TMB Blotting Plus; TMB substrate; TMB
Beilstein Registry Number:
2808541
MDL Number:
MFCD00007748
Product Description

TMB is a chromogenic substrate for Horse Radish Peroxidase (HRP). MP Biolomedicals TMB Solution is a convenient, non toxic and ready to use stable chromogen solution suitable for peroxidase and pseudo-peroxidase reactions in ELISA, it provides consistent performance and sensitivity to deliver reproducible results

Application Notes

Metabolism of the TMB substrate by HRP creates a soluble bluish reaction product that may be quantified by measuring absorbance at 370 or 665 nm. Reaction can be stopped by addition of 1 volume of 1N hydrocloric or sulfurc acid to yield a yellow reaction product that can be measured by absorbance at 450 nm (within one hour).

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

ELISA

Specifications
SKU 02152346-CF
Alternate Names BM blue; Sure Blue TMB; TMB Blotting Plus; TMB substrate; TMB
Application Notes Metabolism of the TMB substrate by HRP creates a soluble bluish reaction product that may be quantified by measuring absorbance at 370 or 665 nm. Reaction can be stopped by addition of 1 volume of 1N hydrocloric or sulfurc acid to yield a yellow reaction product that can be measured by absorbance at 450 nm (within one hour).
Base Catalog Number 152346
Beilstein Registry Number 2808541
Concentration 1.25 mM
Format Ready-to-use 1.25 mmol/liter stabilized chromogen solution.
Hazard Statements H315-H319-H335
Personal Protective Equipment Dust mask type , Eyeshields, Faceshields, Gloves
RTECS Number DV2300000
Safety Symbol GHS07
Solubility Soluble in water
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Documents

Certificate of Analysis (COA)