SPINeasy® DNA Kit for Tissue & Bacteria (With Lysing Matrix), 50 preps

SPINeasy DNA Kit for Tissue and Bacteria is a high-performance genomic DNA (gDNA) extraction kit based on silica-membrane spin-column technology. This kit enables quick isolation of gDNA from various animal tissues, blood, cultured cells and bacteria, typically in less than 30 minutes. The use of our specially formulated Lysis Buffer GD and Lysing Matrix A in combination with FastPrep® Instruments from MP Biomedicals enables highly efficient lysis of various types of samples within seconds. Provided in the kit, Column GD and kit buffers are designed to deliver gDNA extracts of high yield and purity, compatible with downstream applications such as PCR, restriction digestion and sequencing.

Advantages of SPINeasy DNA Kit for Tissue & Bacteria (With Lysing Matrix)

Convenient and fast isolation of pure DNA

SPINeasy DNA Kit for Tissue & Bacteria (with Lysing Matrix) is designed with optimal lysis process for simple, straightforward isolation of genomic DNA (gDNA) from various types of tissues and bacteria. The gDNA extracted is of better purity when compared to the other DNA kits available. The purity of the extracted DNA was determined with spectrophotometric analysis by measuring and calculating the ratio of the absorbance at 260 nm and 280 nm (A260/A280). Generally, purity is considered good with A260/A280 ratio around 1.7- 2.0.

Note: The gDNA extracted using SPINeasy DNA kit for Tissue & Bacteria (with Lysing Matrix) (in blue bars) successfully achieved A260/A280 ratio within 1.7-2.0 across various types of tissues and bacteria.

The purity of the gDNA extracted was also determined using spectrophotometric analysis and calculating the ratio of absorbance at 260 nm and 230 nm. A ratio of 260nm to 230nm can help evaluate the level of salt carryover in the purified DNA. Generally, purity is considered good with A260/A230 ratio of 1.0 or above 1.0. A low ratio can indicate the presence of contaminant such as organic compounds or chaotropic salts in the purified DNA.

Remarks: The gDNA extracted with SPINeasy DNA kit for Tissue & Bacteria (with Lysing Matrix) (in blue bars) have A260/A230 ratios above 1.0 across the types of tissues and bacteria tested.

Higher yield and integrity of gDNA extracted

Consistent high yields were obtained using SPINeasy DNA Kit for Tissue & Bacteria (with Lysing Matrix) along with high purity. Also, integrity of the extracted gDNA was examined by the absence of visible shearing or smearing of band during gel electrophoresis.

M: DL 15000 DNA ladder; Lane 1: Heart; Lane 2: Liver; Lane 3: Spleen; Lane 4: Lung; Lane 5: Kidney; Lane 6: Rabbit blood; Lane 7: Chicken blood; Lane 8: Staphylococcus aureus; Lane 9: Escherichia coli

Note: SPINeasy DNA Kit for Tissue & Bacteria (with Lysing Matrix) obtained intact bands of extracted DNA.

Note: SPINeasy DNA Kit for Tissue & Bacteria (with Lysing Matrix) (in blue bars) consistently achieved higher yields across various types of tissues and bacteria as compared to competitor's DNA kits.

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Application Notes

Deliver gDNA extracts of high yield and purity from animal tissues, blood, cultured cells and bacteria that are compatible with downstream applications such as PCR, restriction digestion and sequencing.

Usage Statement

Research Use Only (RUO).

Key Applications

Genomics, Molecular Biology, PCR, qPCR, NGS