Sodium N-tetradecyl sulfate, 25 g
Sodium N-Tetradecyl Sulfate
Nonidet® P40 (substitute), 5 mL
Nonidet P-40 is a non-ionic detergent used in the pre-treatment solution of slides prior to in situ hybridization. The detergent helps to reduce background or non-specific binding of a probe by reducing debris. NP40 can also be used in the aqueous post-hybridization washing solution and helps to provide optimum washing conditions.
Polysorbate 20, molecular biology reagent