DNA extraction of soil from earthworm intestinal contents with SPINeasy DNA Kit for Soil (Cat. No. 116530050)
Picture source: Institute of Urban Environment, Chinese Academy of Sciences
Gutted by soil DNA quality in Earthworm intestinal microbiome
A study to examine the ecological role of earthworms and the contents in their gut was stumped by constraints of sample preparation. The DNA extraction results achieved by the researcher, using their kit, was relatively low, with DNA yield of 37.2 ng/μl, the absorbance ratio of 260/280 was 1.796, and the purity was only at 0.044. The yield was insufficient for subsequent amplicon sequencing and high-throughput fluorescent PCR. The researcher would need to repeat the sample preparation to improve on the DNA yield from the unique soil sample comprising of mostly earthworm intestinal content and the surrounding soil in the diet
MP Biomedicals stood our grounds and offered SPINeasy DNA Kit for Soil (Cat. No. 116530050) to address the DNA yield issue faced by the researcher.
Overview
Sample Name:
Intestinal soil from earthworm guts and surroundings execretions
Sample Type:
Featured Materials:
SPINeasy® DNA Pro Kit for Soil
High Performance kit for isolation of bacterial, fungal, plant and animal genomic DNA from soil and other environmental samples
Methods
Sample was prepared in order of protocol in accordance to product guidelines found here.
Based on MP company’s technical advices, we optimized experiment conditions:
Outcome
Trial results data
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Conclusion
Key takeaways:
Based on MP company’s technical advice, we optimized the experiment conditions and both the DNA yield and purity were improved. DNA extracted from the intestinal content was 87ng/uL, absorbance ratio 260/230 is 1.4, and the DNA purity can meet downstream experiment requirements.
Looking To Try Our Extraction Kits?MP Biomedicals is now offering Free Trial Pack Application for the SPINeasy® DNA Pro Kit for Soil (5 Preps) to any valid research scientists. |
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