Creatinine amidohydrolase, 150 units/mg solid
SKU: 02153491-CF
Creatinine Amidohydrolase
Application Notes
This enzyme is useful for enzymatic determination of creatinine when coupled with creatine amidinohydrolase, sarcosine dehydrogenase or sarcosine oxidase and formaldehyde dehydrogenase in clinical analysis.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
| SKU | 02153491-CF |
| Application Notes | This enzyme is useful for enzymatic determination of creatinine when coupled with creatine amidinohydrolase, sarcosine dehydrogenase or sarcosine oxidase and formaldehyde dehydrogenase in clinical analysis. |
| Base Catalog Number | 153491 |
| Biochemical Physiological Actions | Creatininase from Pseudomonas sp. is a homohexameric enzyme with a molecular mass of 28.4 kDa per subunit. It is a cyclic amidohydrolase catalysing the reversible conversion of creatinine to creatine. Each monomer contains a binuclear zinc centre near the C termini of the ß-strands and the N termini of the main a-helices. These zinc ions indicate the location of the active site. |
| CAS # | 9025-13-2 |
| EC Number | 232-786-8 |
| Format | Powder |
| Isoelectric Point | 4.7 |
| Personal Protective Equipment | Eyeshields, Gloves, half-mask respirator |
| Source | Pseudomonas sp. |
| Specific Activity | 150 units/mg solid |
| Unit Definition | One unit causes the formation of one µmole of creatinine-picrate per minute at pH 7.5 at 37°C. |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |