3,3' Diaminobenzidine tetrahydrochloride, 100 mg
In stock
SKU: 0215082680
Specifications
| SKU | 0215082680 |
| Alternate Names | DAB |
| Application Notes | 3,3′-Diaminobenzidine tetrahydrochloride (DAB) is a horseradish peroxidase substrate suitable for use in immunoblotting and immunohistological staining procedures. This substrate produces an insoluble, brown end product, which can be observed visually. |
| Base Catalog Number | 15082680 |
| Beilstein Registry Number | 1212988 |
| CAS # | 7411-49-6 |
| EC Number | 231-018-9 |
| Hazard Statements | H341-H350 |
| Melting Point | > 300 °C (Lit.) |
| Molecular Formula | C12H14N4*4HCl |
| Molecular Weight | 360.10 |
| Pack Size | 100 mg |
| Personal Protective Equipment | Eyeshields, Gloves, respirator filter |
| Preparation Method | Typical Preparation of solutions for Peroxidase Detection : Dissolve 12 mg of DAB in 1.0 mL 50 mM Tris buffer, pH 7.3 (or inject 8.3 mL of buffer into serum vials if using #150826). Working size aliquots may be stored for 6 months at -20 °C. The stock solution is diluted 1:20 with 50 mM Tris buffer pH 7.3 just before developing the immunoblot. To 10 mL add 10 uL 30% hydrogen peroxide. Add 10 mL of this substrate solution per 15 x 15 cm2 membrane. Develop the blot at room temperature with agitation until the bands are suitably dark. This typically requires 1-5 min. Stop the reaction by rinsing away the substrate with PBS. |
| RTECS Number | DV8753000 |
| Safety Symbol | GHS08 |
| Solubility | Easily soluble in water. The aqueous solution is unstable and can be used only for a few days even if it is kept in a refrigerator. 1% aqueous solution is dark and turns reddish when exposed to light. |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
Documents
Certificate of Analysis (COA)
Datasheet
Datasheet
Powered by Bioz