3,3',5,5'-Tetramethylbenzidine

Name: 3,3',5,5'-Tetramethylbenzidine
SKU: 02190277-CF
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SKU: 02190277-CF

Key features and details

3,3',5,5'-Tetramethylbenzidine

Synonyms

TMB; TMB substrate; BM blue; Sure Blue TMB; TMB Blotting Plus

CAS Number:

54827-17-7

Molecular Formula:

C16H20N2

Molecular Weight:

240.35 g/mol

Beilstein Registry Number:

2808541

EC Number:

259-364-6

MDL Number:

MFCD00007748

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Product Description

3,3',5,5'-Tetramethylbenzidine

Application Notes

3,3',5,5'-Tetramethylbenzidine is suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases. It is useful in gel staining procedure for low levels of home-associated peroxidases and for enzyme immunoassay of horseradish peroxidase.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Peroxidase substrate suitable for use in ELISA

Specifications

SKU	02190277-CF
Alternate Names	TMB; TMB substrate; BM blue; Sure Blue TMB; TMB Blotting Plus
Application Notes	3,3',5,5'-Tetramethylbenzidine is suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases. It is useful in gel staining procedure for low levels of home-associated peroxidases and for enzyme immunoassay of horseradish peroxidase.
Base Catalog Number	190277
Beilstein Registry Number	2808541
CAS #	54827-17-7
EC Number	259-364-6
Format	Powder
Hazard Statements	H315-H319-H335
Melting Point	334 to 336° F (NTP, 1992)
Molecular Formula	C16H20N2
Molecular Weight	240.35 g/mol
Personal Protective Equipment	Dust mask, Eyeshields, Faceshields, Gloves
Purity	≥99%
RTECS Number	DV2300000
Safety Symbol	GHS07
Solubility	less than 1 mg/mL at 68° F (NTP, 1992)
Typical Working Concentration	Solutions for HRP substrate: Dissolve 1.5 mg TMB in 0.1 mL of DMSO. Then add 9.9 ml of 0.1 M sodium acetate (pH 6.0). Finally, add hydrogen peroxide to a concentration of 0.01% and filter through a Whatman #1 paper filter. Prepare solutions fresh daily. Discard any remaining solution at the end of the day.
Usage Statement	Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.