HRP IgG Goat Anti-Rat IgG (H&L), 2 mL
SKU: 0855770
Peroxidase-Conjugated Goat igg Fraction to Rat igg (Whole Molecule) is the lyophilized powder of horseradish peroxidase (HRP)-conjugated goat IgG fraction to rat IgG (whole molecule) and buffer salts.
Application Notes
Peroxidase-Conjugated Goat igg Fraction to Rat igg (Whole Molecule) is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies or their fragments are recommended to avoid non-specific binding from inherent antibodies of host animals. This product is also used in immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Immunohistochemistry | Immunoblotting |ELISA
| SKU | 0855770 |
| Antibody Type | Polyclonal Antibody |
| Application Notes | Peroxidase-Conjugated Goat igg Fraction to Rat igg (Whole Molecule) is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies or their fragments are recommended to avoid non-specific binding from inherent antibodies of host animals. This product is also used in immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections. |
| Base Catalog Number | 55770 |
| Concentration | Total Protein: 5.5 mg/mL Titer: 1:120;000 |
| Conjugate | HRP |
| Format | lyophilized |
| Formulation Details | Lyophilized |
| Grade | Molecular Grade |
| Host | Goat |
| Pack Size | 2 mL |
| Preparation Method | Antibody and highly purified HRP (Rz>3.0) are conjugated under defined conditions to obtain optimally labeled product. Conjugated protein is purified by salt fractionation. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.01% thimerosal, adjusted to standard titer, filtered through a 0.22 µm filter, vialed and lyophilized. |
| Purity | Purified |
| Solubility | Soluble in water |
| Species Reactivity | Rat |
| Specificity | It shows reactivity to rat IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG; some antibodies are expected to bind light chain sites common to all immunoglobulins. Antibody activity to non-immunoglobulin serum proteins is not present. |
| Typical Working Concentration | SUGGESTED WORKING DILUTIONS: 1:500 - 1:4,000 for Tissue Staining 1:1,000 - 1;8,000 for Immunoblotting 1:2,000 - 1:16,000 for Elisa |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
![MP COA [55770] lot ID: 39649](https://www.mpbio.com/static/version1781469166/frontend/Mpbio/luma/en_US/images/document.png "MP COA [55770] lot ID: 39649")
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