Product is the lyophilized powder of goat antiserum to human alpha-1-antitrypsin and buffer salts. The antiserum is prepared by hyperimmunizing goats with highly purified human alpha-1-antitrypsin. Antiserum is delipidated and made specific by solid-phase absorption. The resulting antiserum is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 µm filter, vialed and lyophilized.
USES: This product is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques.
SUGGESTED WORKING DILUTIONS (It is recommended that each lab obtain their own optimal working dilution for each assay):
1:50 - 1:200 for Tissue Staining
1:100 - 1:500 for Immunoblotting
1:400 - 2,000 for ELISA
RECONSTITUTION: Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material.
Application Notes
This product is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Immunoassays | Immunoelectrophoresis | Immunodiffusion | Immunoprecipitation
SKU | 0855111 |
Antibody Type | Polyclonal Antibody |
Application Notes | This product is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. |
Base Catalog Number | 55111 |
Concentration | Antibody Protein: 5 mg/ml |
Conjugate | Unconjugated |
Format | lyophilized |
Formulation Details | Lyophilized |
Grade | Molecular Grade |
Host | Goat |
Immunogen | Human Alpha-1-antitrypsin |
Pack Size | 2 mL |
Solubility | Reconstitution: Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material. |
Species Reactivity | Human |
Specificity | It shows reactivity to human alpha-1-antitrypsin; cross-reactivity to other species may exist. Antibody activity to other serum proteins is not present. |
Typical Working Concentration | 1:50 - 1:200 for Tissue Staining 1:100 - 1:500 for Immunoblotting 1:400 - 2,000 for Elisa |
Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |