MagBeads Plasmid Miniprep Kit (Ready-to-Use for MPure-96™)

Name: MagBeads Plasmid Miniprep Kit (Ready-to-Use for MPure-96™)
SKU: 117040200
Price: 1353 CHF

CHF 1,353.00

In stock

SKU: 117040200

Key features and details

Yields 2–20 µg of plasmid DNA from 0.5–5 mL of overnight E. coli cultures.
Recommended to be used with MPure-96™ aNAP system (Cat. No. EMC044)
Suitable for high and low copy number plasmids.

Packaging size:

96 Preps

Qty

Product Description

The Ready-to-Use MagBeads Plasmid Miniprep Kit offers a scalable and automatable purification method using magnetic bead technology, suitable for extracting both high and low copy number plasmids. This kit efficiently yields plasmid DNA (2–20 µg) from 0.5–5 mL of overnight E. coli cultures, leveraging paramagnetic beads for rapid processing. Its magnetic technology and Ready-to-use format enhance user-friendliness, making it ideal for high-throughput automated nucleic acid purification.

The kit includes components for cell lysis and neutralization. Bacterial cells are first resuspended in Resuspension Buffer. Following resuspension, the cells are lysed with Alkaline Lysis Buffer to release plasmid DNA into the solution. Contaminants such as proteins and chromosomal DNA are precipitated by the Neutralization Buffer. The supernatant, which contains the plasmid DNA, binds to the magnetic particles, and a subsequent wash with an alcohol-containing buffer effectively removes impurities, resulting in purified plasmid DNA.

This kit is compatible with the MPure-32™ aNAP System, or other compatible high throughput instruments, facilitating a faster extraction process that takes approximately 40–50 minutes.

Performance

Figure 1. Agarose gel electrophoresis of (a) pWPI and (b) pBBRMCS-2 plasmid extracted using MagBeads Plasmid Miniprep Kit and Competitor O. Lane M: DNA marker. Lane 1 - 2: Manual extraction using MagBeads Plasmid Miniprep kit. Lane 3 - 4: Manual extraction using Competitor O kit.

Figure 2. Downstream applications of plasmids extracted using the MagBeads Plasmid Miniprep Kit (as shown by agarose gel electrophoresis):

(a) PCR amplification

Lane 1–2: PCR products using manually extracted plasmid DNA as template.

Lane 3: Negative control (no template).

Lane M: DNA marker.

(b) Restriction digestion with EcoRI and NotI

Lane 1: Uncut plasmid.

Lane 2: Plasmid digested with EcoRI and NotI.

Lane M: DNA marker.

Application Notes

The MagBeads Plasmid Miniprep Kit (Ready-to-Use for MPure-96™) was designed for rapidly isolating high-quality high and low copy number plasmids using Magnetic Beads automation technology.

Usage Statement

Research Use Only (RUO)

Key Applications

PCR, restriction digestion

Specifications

SKU	117040200
Application Notes	The MagBeads Plasmid Miniprep Kit (Ready-to-Use for MPure-96™) was designed for rapidly isolating high-quality high and low copy number plasmids using Magnetic Beads automation technology.
Base Catalog Number	7040200
Product Line	MagBeads
Components	Resuspension Buffer MN1 (26 mL), Alkaline Lysis Buffer MN2 (26 mL), Neutralization Buffer MN3 (37 mL), Binding Buffer MN (96 Preps), Wash Buffer MN (96 Preps), Elution Buffer MN (96 Preps), Elution Buffer MN (8 mL), 96 Spin Tips (1 ea), RNase A (260 µL), Indicator Solution (130 µL).
Format	Kit
Pack Size	96 Preps
Personal Protective Equipment	Safety Goggles/Faceshield; Gloves
Storage	Store the Elution Buffer MN plate and Resuspension Buffer MN1 after adding RNase A at 2-8°C, the remaining kit contents should be stored at 15-25°C.
Usage Statement	Research Use Only (RUO)