The MagBeads Plasmid Miniprep Kit offers a scalable and automatable purification method using magnetic bead technology, suitable for extracting both high and low copy number plasmids. This kit efficiently yields plasmid DNA (2 - 20 µg) from 0.5 - 5 mL of overnight E. coli cultures, utilizing paramagnetic beads for rapid processing. Its magnetic technology enhances user-friendliness, making it ideal for both small sample sizes and high-throughput automated nucleic acid purification.
The kit includes components for cell lysis and neutralization. Bacterial cells are first resuspended in Resuspension Buffer. Following resuspension, the cells are lysed with Alkaline Lysis Buffer to release plasmid DNA into the solution. Contaminants such as proteins and chromosomal DNA are precipitated by the Neutralization Buffer. The supernatant, which contains the plasmid DNA, binds to the magnetic particles, and a subsequent wash with an alcohol-containing buffer effectively removes impurities, resulting in purified plasmid DNA.
This kit is compatible with the MPure-32™ or MPure-96™ aNAP System, or other compatible high-throughput instruments, facilitating a faster extraction process that takes approximately 40 - 50 minutes.
Performance
Figure 1. Agarose gel electrophoresis of (a) pWPI and (b) pBBRMCS-2 plasmid extracted using MagBeads Plasmid Miniprep Kit and Competitor O. Lane M: DNA marker. Lane 1 - 2: Manual extraction using MagBeads Plasmid Miniprep kit. Lane 3 - 4: Manual extraction using Competitor O kit.
Figure 2. Downstream applications of plasmids extracted using the MagBeads Plasmid Miniprep Kit (as shown by agarose gel electrophoresis):
(a) PCR amplification
Lane 1–2: PCR products using manually extracted plasmid DNA as template.
Lane 3: Negative control (no template).
Lane M: DNA marker.
(b) Restriction digestion with EcoRI and NotI
Lane 1: Uncut plasmid.
Lane 2: Plasmid digested with EcoRI and NotI.
Lane M: DNA marker.
Application Notes
The MagBeads Plasmid Miniprep Kit was designed for rapidly isolating high-quality high and low copy number plasmids using Magnetic Beads automation technology.
Key Applications
PCR, restriction digestion
| SKU | 116587050 |
| Application Notes | The MagBeads Plasmid Miniprep Kit was designed for rapidly isolating high-quality high and low copy number plasmids using Magnetic Beads automation technology. |
| Base Catalog Number | 6587050 |
| Product Line | MagBeads |
| Format | Kit |
| Pack Size | 50 Preps |
| Personal Protective Equipment | Safety Goggles/Faceshield; Gloves |
| Sample Type | Plasmid Prep |
| Storage | Store the Magnetic Beads R and Resuspension Buffer MN1 after adding RNase A at 2 - 8˚C, the remaining kit contents should be stored at 15-25°C. |
Powered by Bioz